Bacterium |
Desulfocucumis palustris DSM 102911
|
7-14 days | 28 °C | anaerobic
|
Increase amount of yeast extract to 0.50 g/l and replace pyruvate with 0.50 g/l Na-acetate added from an anoxic sterile stock solution. Use 5% (v/v) as inoculum.
|
DSMZ
BacDive
|
Bacterium |
Lactivibrio alcoholicus DSM 24196
|
3-7 days | 37 °C | anaerobic
|
Increase amount of yeast extract to 0.20 g/l and replace pyruvate with 0.18 g/l D-glucose added from an anoxic stock solution sterilized by filtration. Use 5% (v/v) as inoculum.
|
DSMZ
BacDive
|
Archaeon |
Methanocella paludicola DSM 17711
|
7-14 days | 37 °C | anaerobic
|
Replace pyruvate with 0.10 g/l Na-acetate and pressurize serum vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
|
DSMZ
BacDive
|
Archaeon |
Methanofollis ethanolicus DSM 21041
|
37 °C | anaerobic
|
Replace pyruvate with 0.92 ml/l ethanol.
|
DSMZ
BacDive
|
Archaeon |
Methanolinea mesophila DSM 23604
|
more than 14 days | 37 °C | anaerobic
|
Replace pyruvate with 0.10 g/l Na-acetate and pressurize serum vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
|
DSMZ
BacDive
|
Archaeon |
Methanolinea tarda DSM 16494
|
more than 14 days | 50 °C | anaerobic
|
Replace pyruvate with 2.72 g/l Na-formate and 0.10 g/l Na-acetate. Supplement medium with 0.08 g/l coenzyme M (2-mercaptoethanesulfonic acid).
|
DSMZ
BacDive
|
Archaeon |
Methanoregula formicica DSM 22288
|
more than 14 days | 30 °C | anaerobic
|
Replace pyruvate with 2.72 g/l Na-formate and 0.10 g/l Na-acetate. Supplement medium with 0.08 g/l coenzyme M (2-mercaptoethanesulfonic acid).
|
DSMZ
BacDive
|
Bacterium |
Oligosphaera ethanolica DSM 24202
|
7-14 days | 37 °C | anaerobic
|
Omit yeast extract and replace pyruvate with 0.18 g/l D-glucose added from an anoxic stock solution sterilized by filtration. Use 10% (v/v) as inoculum.
|
DSMZ
BacDive
|
Bacterium |
Pelotomaculum propionicicum DSM 15578
|
more than 14 days | 37 °C | anaerobic
|
Replace pyruvate with 2.00 g/l Na-propionate.
|
DSMZ
BacDive
|
Bacterium |
Pelotomaculum schinkii DSM 15200
|
more than 14 days | 37 °C | anaerobic
|
Replace pyruvate with 2.00 g/l Na-propionate.
|
DSMZ
BacDive
|
Bacterium |
Pelotomaculum sp. DSM 16145
|
more than 14 days | 37 °C | anaerobic
|
Replace pyruvate with 2.00 g/l Na-propionate.
|
DSMZ
BacDive
|
Bacterium |
Pelotomaculum terephthalicicum DSM 16121
|
more than 14 days | 37 °C | anaerobic
|
Replace pyruvate with 1.50 g/l Na-2,5-dihydroxybenzoate.
|
DSMZ
BacDive
|
Bacterium |
Pelotomaculum thermopropionicum DSM 13744
|
55 °C | anaerobic
|
|
DSMZ
BacDive
|
Bacterium |
Pelotomaculum thermopropionicum DSM 13752
|
7-14 days | 55 °C | anaerobic
|
Replace pyruvate with 0.92 ml/l ethanol.
|
DSMZ
BacDive
|
Bacterium |
Sporotomaculum syntrophicum DSM 14795
|
37 °C | anaerobic
|
Replace pyruvate with 0.86 g/l Na-crotonate.
|
DSMZ
BacDive
|
Bacterium |
Sporotomaculum syntrophicum DSM 14947
|
37 °C
|
Replace pyruvate with 0.72 g/l Na-benzoate.
|
DSMZ
BacDive
|
Bacterium |
Syntrophomonas palmitatica DSM 18709
|
3-7 days | 37 °C | anaerobic
|
Replace pyruvate with 0.86 g/l Na-crotonate and increase concentration of yeast extract to 0.50 g/l.
|
DSMZ
BacDive
|
Bacterium |
Syntrophorhabdus aromaticivorans DSM 17771
|
more than 14 days | 37 °C | anaerobic
|
Supplement medium with 1.42 g/l Na2SO4 and replace pyruvate with 0.41 g/l 4-hydroxybenzoic acid added from a sterile, neutralized and anoxic stock solution. Increase concentration of yeast extract to 0.20 g/l.
|
DSMZ
BacDive
|
Bacterium |
Syntrophus aciditrophicus DSM 26646
|
3-7 days | 35 °C | anaerobic
|
Replace pyruvate with 0.86 g/l Na-crotonate and increase concentration of yeast extract to 0.50 g/l.
|
DSMZ
BacDive
|