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843 HELMUTKOENIGIA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 13.958 -
Trehalose 1.893 5.531
Maltose x H2O 1.795 5.243
Sucrose 1.696 4.954
Cellobiose 1.696 4.954
Na2CO3 1.496 14.11
NaCl 0.997 17.06
Trypticase peptone 0.997 -
D-Xylose 0.748 4.981
KCl 0.499 6.687
DL-Dithiothreitol 0.399 2.585
MgCl2 x 6 H2O 0.399 1.962
NH4Cl 0.299 5.592
KH2PO4 0.199 1.465
Na2SO4 0.15 1.053
CaCl2 x 2 H2O 0.1 0.678
Thiamine pyrophosphate 0.05 0.108
Pyridoxal hydrochloride 0.005 0.024
Pyridoxal phosphate 0.005 0.02
HCl 0.002 0.068
FeCl2 x 4 H2O 0.001 0.008
Haemin 0.001 0.002
Ca-folinate 1.0e-3 0.002
Coenzyme A 1.0e-3 0.001
FAD 1.0e-3 0.001
ß-NAD 1.0e-3 -
NaOH 5.0e-4 0.012
Nicotinamide 5.0e-4 0.004
Sodium resazurin 5.0e-4 0.002
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.3e-4
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
Calcium pantothenate 1.0e-4 2.1e-4
Vitamin B12 1.0e-4 7.4e-5
p-Aminobenzoic acid 8.0e-5 5.8e-4
ZnCl2 7.0e-5 5.1e-4
Folic acid 5.0e-5 1.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
D-(+)-biotin 2.0e-5 8.2e-5
Riboflavin 1.0e-5 2.6e-5
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Distilled water - -
Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 30 ml Sugar mix
  • 50 ml Yeast autolysate
  • 1 ml Seven vitamins solution
  • 20 ml Co-factors solution
Gas phase:
  • 80 % N2
  • 20 % CO2