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Current criteria: α-D-Glucose monohydrate

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1566 HAHA100 V MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 26.37 451.232
MgSO4 x 7 H2O 6.8 27.589
MgCl2 x 6 H2O 5.67 27.889
CaCl2 x 2 H2O 1.47 9.999
KCl 0.72 9.658
NaHCO3 0.168 2
KBr 0.1 0.84
Cellobiose 0.1 0.292
Casamino acids 0.1 -
Yeast extract 0.1 -
Tryptone peptone 0.1 -
α-D-Glucose monohydrate 0.1 -
H3BO3 0.02 0.323
KH2PO4 0.02 0.147
SrCl2 0.02 0.126
Na2-EDTA 0.01 0.031
Nicotinic acid 0.005 0.041
Pyridoxine hydrochloride 0.005 0.024
Thiamine-HCl x 2 H2O 0.005 0.013
Calcium pantothenate 0.005 0.01
NaF 0.003 0.071
FeCl2 x 4 H2O 0.003 0.015
NaOH 1.0e-3 0.025
Riboflavin 1.0e-3 0.003
NH4Cl 8.0e-4 0.015
CoCl2 x 6 H2O 3.8e-4 0.003
MnCl2 x 4 H2O 2.0e-4 0.001
ZnCl2 1.4e-4 0.001
Vitamin B12 1.0e-4 7.4e-5
Na2MoO4 x 2 H2O 7.2e-5 3.0e-4
NiCl2 x 6 H2O 4.8e-5 3.7e-4
CuCl2 x 2 H2O 3.4e-5 2.0e-4
Folic acid 2.0e-5 4.5e-5
Biotin 1.0e-5 4.1e-5
Na2WO4 x 2 H2O 8.0e-6 2.4e-5
Na2SeO3 x 5 H2O 6.0e-6 2.3e-5
Distilled water - -
Solutions:
  • 10 ml Standard vitamin solution
  • 2 ml Micronutrient solution SL 8
  • 2 ml Selenite-tungstate solution