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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Bicine
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1381 ½ S° HELIORESTIS MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 2.5 | 29.759 |
| Na2CO3 | 2.5 | 23.588 |
| Bicine | 1.63 | 9.99 |
| Na-acetate | 1 | 12.19 |
| Na-pyruvate | 1 | 9.087 |
| NH4Cl | 0.5 | 9.348 |
| KH2PO4 | 0.5 | 3.674 |
| Na2S x 9 H2O | 0.5 | 2.082 |
| Na2S2O3 x 5 H2O | 0.5 | 2.015 |
| MgSO4 x 7 H2O | 0.2 | 0.811 |
| Yeast extract | 0.1 | - |
| CaCl2 | 0.075 | 0.676 |
| EDTA | 0.005 | 0.018 |
| FeCl2 x 4 H2O | 0.002 | 0.008 |
| CoCl2 x 6 H2O | 1.9e-4 | 0.001 |
| Na2MoO4 x 2 H2O | 1.9e-4 | 7.8e-4 |
| MnCl2 x 4 H2O | 1.0e-4 | 5.1e-4 |
| ZnCl2 | 7.0e-5 | 5.1e-4 |
| VOSO4 x 2 H2O | 3.0e-5 | 1.5e-4 |
| NiCl2 x 6 H2O | 2.5e-5 | 1.9e-4 |
| Vitamin B12 | 2.0e-5 | 1.5e-5 |
| CuCl2 x 2 H2O | 1.7e-5 | 1.0e-4 |
| H3BO3 | 6.0e-6 | 9.7e-5 |
| Na2WO4 x 2 H2O | 2.0e-6 | 6.1e-6 |
| NaHSeO3 | 2.0e-6 | - |
| Deionized water | - | - |
| Distilled water | - | - |
Solutions:
- 1 ml Trace elements