Name: ½ S° HELIORESTIS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1381 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
EDTA (1%)	1.000	ml	0.01	0.034
KH₂PO₄	0.500	g	0.5	3.674
NH₄Cl	0.500	g	0.5	9.348
Bicine	1.630	g	1.63	9.99
MgSO₄ x 7 H₂O	0.200	g	0.2	0.811
CaCl₂	0.075	g	0.075	0.676
Na₂CO₃	2.500	g	2.5	23.588
NaHCO₃	2.500	g	2.5	29.759
Na-acetate	1.000	g	1	12.19
Yeast extract	0.100	g	0.1	-
Na₂S x 9 H₂O	0.250	g	0.5	2.082
Na-pyruvate	1.000	g	1	9.087
Trace elements	1.000	ml	-	-
Vitamin B₁₂	20.000	µg	2.0e-5	1.5e-5
Distilled water	1000.000	ml	-	-
1 Prepare under N₂ gas and add vitamin B₁₂, NaHCO₃, Na₂CO₃ and sulfide as single sterile stock solutions to sterile medium after autoclaving. Adjust pH to 9 - 9.5.

Trace elements #

1000

Solution occurs in 2 other media (first defined in Medium 1147).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
EDTA	5.2	g	5.2	17.794
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
MnCl₂ x 4 H₂O	0.1	g	0.1	0.505
FeCl₂ x 4 H₂O	1.5	g	1.5	7.545
H₃BO₃	6.0	mg	0.006	0.097
CuCl₂ x 2 H₂O	17.0	mg	0.017	0.1
Na₂MoO₄ x 2 H₂O	188.0	mg	0.188	0.777
NiCl₂ x 6 H₂O	25.0	mg	0.025	0.193
ZnCl₂	70.0	mg	0.07	0.514
VOSO₄ x 2 H₂O	30.0	mg	0.03	0.151
Na₂WO₄ x 2 H₂O	2.0	mg	0.002	0.006
NaHSeO₃	2.0	mg	0.002	-
Deionized water	1000.0	ml	-	-

All strains for this medium:	3
Strains with modifications:	1

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