DSMZ 1381 . ½ S° HELIORESTIS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA
(1%)
1.000 ml 0.01 0.034
KH2PO4 0.500 g 0.5 3.674
NH4Cl 0.500 g 0.5 9.348
Na2S2O3 x 5 H2O 0.500 g 0.5 2.015
Bicine 1.630 g 1.63 9.99
MgSO4 x 7 H2O 0.200 g 0.2 0.811
CaCl2 0.075 g 0.075 0.676
Na2CO3 2.500 g 2.5 23.588
NaHCO3 2.500 g 2.5 29.759
Na-acetate 1.000 g 1 12.19
Yeast extract 0.100 g 0.1 -
Na2S x 9 H2O 0.500 g 0.5 2.082
Na-pyruvate 1.000 g 1 9.087
Trace elements 1.000 ml - -
Vitamin B12 20.000 µg 2.0e-5 1.5e-5
Distilled water 1000.000 ml - -
1 Prepare under N2 gas and add vitamin B12, NaHCO3, Na2CO3 and sulfide as single sterile stock solutions to sterile medium after autoclaving. Adjust pH to 9 - 9.5.
Solution occurs in 2 other media (first defined in Medium 1147).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.2 g 5.2 17.794
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
MnCl2 x 4 H2O 0.1 g 0.1 0.505
FeCl2 x 4 H2O 1.5 g 1.5 7.545
H3BO3 6.0 mg 0.006 0.097
CuCl2 x 2 H2O 17.0 mg 0.017 0.1
Na2MoO4 x 2 H2O 188.0 mg 0.188 0.777
NiCl2 x 6 H2O 25.0 mg 0.025 0.193
ZnCl2 70.0 mg 0.07 0.514
VOSO4 x 2 H2O 30.0 mg 0.03 0.151
Na2WO4 x 2 H2O 2.0 mg 0.002 0.006
NaHSeO3 2.0 mg 0.002 -
Deionized water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.0 - 9.5
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.5 29.759
Na2CO3 2.5 23.588
Bicine 1.63 9.99
Na-acetate 1 12.19
Na-pyruvate 1 9.087
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Cultivation metadata from 3 strains BacDive

phyla 2 3 strains
oxygen anaerobe 1 strain
temperature mesophilic 3 strains