Main sol. 1381
Internal Solution-ID: S2801
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
EDTA
(1%) |
1.000 | ml | 0.01 | 0.034 | |
| KH2PO4 | 0.500 | g | 0.5 | 3.674 | |
| NH4Cl | 0.500 | g | 0.5 | 9.348 | |
| Bicine | 1.630 | g | 1.63 | 9.99 | |
| MgSO4 x 7 H2O | 0.200 | g | 0.2 | 0.811 | |
| CaCl2 | 0.075 | g | 0.075 | 0.676 | |
| Na2CO3 | 2.500 | g | 2.5 | 23.588 | |
| NaHCO3 | 2.500 | g | 2.5 | 29.759 | |
| Na-acetate | 1.000 | g | 1 | 12.19 | |
| Yeast extract | 0.100 | g | 0.1 | - | |
| Na2S x 9 H2O | 0.250 | g | 0.5 | 2.082 | |
| Na-pyruvate | 1.000 | g | 1 | 9.087 | |
| Trace elements | 1.000 | ml | - | - | |
| Vitamin B12 | 20.000 | µg | 2.0e-5 | 1.5e-5 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Prepare under N2 gas and add vitamin B12, NaHCO3, Na2CO3 and sulfide as single sterile stock solutions to sterile medium after autoclaving. Adjust pH to 9 - 9.5. | |||||
Media with this solution
| 1381 | ½ S° HELIORESTIS MEDIUM |