Name: RV5 MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1147 #

Equipment needed: Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
EDTA	10.00	mg	0.01	0.034
MgSO₄ x 7 H₂O	200.00	mg	0.2	0.811
CaCl₂ x 2 H₂O	75.00	mg	0.075	0.51
NaCl	15.00	g	15	256.674
NH₄Cl	0.52	g	0.52	9.721
DL-Malic acid	4.00	g	4	29.831
K₂HPO₄	1.00	g	1	5.741
BICINE buffer (Sigma)	10.00	mM	1.632	10
Trace elements	1.00	ml	-	-
Distilled water	1000.00	ml	-	-
1 Filter-sterilize and add after autoclaving:
Thiamine	1.00	mg	1.0e-3	0.004
Vitamin B₁₂	20.00	µg	2.0e-5	1.5e-5
Biotin	30.00	µg	3.0e-5	1.2e-4
2 The pH of the medium was adjusted to 9.5 by addition of NaOH and then sterilized by autoclaving. Before inoculating medium RV5, 0.1% (w/v) NaHCO₃ was added to tubes, bottles, or molten media from a 5% (w/v) filter-sterilized stock solution. For routine culture, medium RV5 supplemented with 0.05% (w/v) Difco yeast extract was used.

Trace elements #

1000

Solution occurs in 2 other media (first defined in Medium 1147).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
EDTA	5.2	g	5.2	17.794
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
MnCl₂ x 4 H₂O	0.1	g	0.1	0.505
FeCl₂ x 4 H₂O	1.5	g	1.5	7.545
H₃BO₃	6.0	mg	0.006	0.097
CuCl₂ x 2 H₂O	17.0	mg	0.017	0.1
Na₂MoO₄ x 2 H₂O	188.0	mg	0.188	0.777
NiCl₂ x 6 H₂O	25.0	mg	0.025	0.193
ZnCl₂	70.0	mg	0.07	0.514
VOSO₄ x 2 H₂O	30.0	mg	0.03	0.151
Na₂WO₄ x 2 H₂O	2.0	mg	0.002	0.006
NaHSeO₃	2.0	mg	0.002	-
Deionized water	1000.0	ml	-	-

All strains for this medium:

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phyla	1	1 strain
temperature	mesophilic	1 strain