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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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Current criteria: CoCl2 x 2 H2O

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748 CENS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Soytone 4 -
Na-pyruvate 2.2 19.992
NH4Cl 1 18.695
K2HPO4 0.9 5.167
KH2PO4 0.6 4.409
Na2S2O3 x 5 H2O 0.5 2.015
MgSO4 x 7 H2O 0.2 0.811
CaCl2 x 2 H2O 0.075 0.51
EDTA 0.004 0.015
FeCl2 x 4 H2O 0.002 0.011
MnCl2 x 4 H2O 8.0e-4 0.004
H3BO3 4.0e-4 0.006
Na2MoO4 x 2 H2O 4.0e-4 0.002
NiCl2 x 6 H2O 2.0e-4 0.002
ZnCl2 2.0e-4 0.001
CoCl2 x 2 H2O 8.0e-5 6.2e-4
CuCl2 x 2 H2O 4.0e-5 2.3e-4
NaVO3 x n H2O 2.0e-5 1.4e-4
Na2SeO3 2.0e-5 1.2e-4
Vitamin B12 2.0e-5 1.5e-5
Biotin 1.5e-5 6.1e-5
Distilled water - -
HCl - -
Solutions:
  • 1 ml True Blue Trace Elements
  • 2 ml Chelated Iron solution