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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: CuCl2 x 6 H2O
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1181 METHYLOCELLA SILVERSTRIS MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2HPO4 x 12 H2O | 0.717 | 5.051 |
| KH2PO4 | 0.272 | 1.999 |
| NaNO3 | 0.2 | 2.353 |
| MgSO4 x 7 H2O | 0.2 | 0.811 |
| NaH2PO4 x 2 H2O | 0.144 | 1.196 |
| CaCl2 x 6 H2O | 0.006 | 0.027 |
| Fe(III)NH4-EDTA | 0.004 | - |
| H3BO3 | 1.5e-5 | 2.4e-4 |
| CaCl2 x 2 H2O | 1.0e-5 | 6.8e-5 |
| ZnSO4 x 7 H2O | 5.0e-6 | 1.7e-5 |
| MnCl2 x 4 H2O | 1.5e-6 | 7.6e-6 |
| Na2MoO4 x 2 H2O | 1.5e-6 | 6.2e-6 |
| CuCl2 x 6 H2O | 5.0e-7 | 3.7e-6 |
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Solutions:
- 0.5 ml Trace element solution
- 5 ml Phosphate buffer
1396 HALOARCHAEAL MEDIUM MH-1
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 249.377 | 4267.22 |
| Agar | 24.938 | 74.146 |
| K2SO4 | 6.234 | 35.777 |
| Casamino acids | 4.988 | - |
| L-Glutamic acid | 2.494 | 16.949 |
| Trisodium citrate x 2 H2O | 2.494 | 8.479 |
| Yeast extract | 2.494 | - |
| NH4Cl | 1.247 | 23.311 |
| KH2PO4 | 1.247 | 9.163 |
| FeSO4 x 6 H2O | 0.005 | 0.033 |
| Na2S2O3 x 5 H2O | 0.005 | 0.02 |
| CaCl2 x 2 H2O | 0.002 | 0.017 |
| CoCl2 x 6 H2O | 7.5e-4 | 0.006 |
| NiCl2 x 6 H2O | 2.5e-4 | 0.002 |
| ZnCl2 | 2.5e-4 | 0.002 |
| MnCl2 x 4 H2O | 2.5e-4 | 0.001 |
| Na2MoO4 x 2 H2O | 2.5e-4 | 0.001 |
| AlCl3 | 1.0e-4 | 7.5e-4 |
| Na2WO4 x 2 H2O | 5.0e-5 | 1.5e-4 |
| H3BO4 | 5.0e-5 | - |
| CuCl2 x 6 H2O | 2.5e-5 | 1.9e-4 |
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Solutions:
- 2 ml Trace Element Solution
1497 HALOARCHAEUM SALINUM MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 200 | 3422.31 |
| MgCl2 x 7 H2O | 40 | 420.122 |
| Agar | 40 | 118.931 |
| K2SO4 | 5 | 28.693 |
| Casamino acids | 4 | - |
| L-Glutamic acid | 2 | 13.593 |
| Trisodium citrate x 2 H2O | 2 | 6.8 |
| Yeast extract | 2 | - |
| NH4Cl | 1 | 18.695 |
| KH2PO4 | 1 | 7.348 |
| FeSO4 | 0.004 | 0.026 |
| Na2S2O3 x 5 H2O | 0.004 | 0.016 |
| CaCl2 x 2 H2O | 0.002 | 0.014 |
| CoCl2 x 6 H2O | 6.0e-4 | 0.005 |
| NiCl2 x 6 H2O | 2.0e-4 | 0.002 |
| ZnCl2 | 2.0e-4 | 0.001 |
| MnCl2 x 4 H2O | 2.0e-4 | 0.001 |
| Na2MoO4 x 2 H2O | 2.0e-4 | 8.3e-4 |
| AlCl3 | 8.0e-5 | 6.0e-4 |
| Na2WO4 x 2 H2O | 4.0e-5 | 1.2e-4 |
| H3BO4 | 4.0e-5 | - |
| CuCl2 x 6 H2O | 2.0e-5 | 1.5e-4 |
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Solutions:
- 500 ml Mineral Salts Solution for solid Medium
- 500 ml Mineral Salts Solution for liquid Medium
- 1 ml Trace Element Solution
- 1 ml Trace Element Solution