Name: HALOARCHAEAL MEDIUM MH-1
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1396 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
L-Glutamic acid	2	g	2.494	16.949
Trisodium citrate x 2 H₂O	2	g	2.494	8.479
KH₂PO₄	1	g	1.247	9.163
NH₄Cl	1	g	1.247	23.311
K₂SO₄	5	g	6.234	35.777
NaCl	200	g	249.377	4267.22
Casamino acids	4	g	4.988	-
Yeast extract	2	g	2.494	-
FeSO₄ x 6 H₂O	4	mg	0.005	0.033
Trace Element Solution	2	ml	-	-
Agar	20	g	24.938	74.146
Distilled water	800	ml	-	-
1 Adjust to pH 4.5 with 40% KOH and sterilize by autoclaving.

Trace Element Solution #

1000

Solution occurs in 1 other media (first defined in Medium 1396).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
CoCl₂ x 6 H₂O	0.30	g	0.3	2.311
MnCl₂ x 4 H₂O	0.10	g	0.1	0.505
ZnCl₂	0.10	g	0.1	0.734
H₃BO₄	0.02	g	0.02	-
Na₂MoO₄ x 2 H₂O	0.10	g	0.1	0.413
NiCl₂ x 6 H₂O	0.10	g	0.1	0.772
CuCl₂ x 6 H₂O	0.01	g	0.01	0.074
AlCl3	0.04	g	0.04	0.3
Na₂WO₄ x 2 H₂O	0.02	g	0.02	0.061
CaCl₂ x 2 H₂O	1.00	g	1	6.802
BaCl2 x 2 H₂O	0.01	g	0.01	0.041
Na₂S₂O₃ x 5 H₂O	2.00	g	2	8.058
Distilled water	1000.00	ml	-	-
1 Adjust to pH 4.0.

All strains for this medium:	2
Strains with modifications:	2

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