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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Li2B4O7
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1493 VERRUCOMICROBIA MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 0.5 | 8.556 |
| NaHCO3 | 0.5 | 5.952 |
| KH2PO4 | 0.4 | 2.939 |
| (NH4)2SO4 | 0.2 | 1.514 |
| MgSO4 x 6 H2O | 0.1 | 0.831 |
| CaCl2 | 0.01 | 0.09 |
| Li2B4O7 | 0.01 | - |
| Na-EDTA | 0.002 | 0.006 |
| FeSO4 x 7 H2O | 0.002 | 0.006 |
| Nitrilotriacetic acid | 0.002 | 0.008 |
| ZnSO4 x 7 H2O | 5.4e-4 | 0.002 |
| Na2SeO4 x 10 H2O | 4.4e-4 | - |
| CuSO4 | 2.1e-4 | 0.001 |
| Fe(NH4)2(SO4)2 x 6 H2O | 2.0e-4 | - |
| MnCl2 | 1.9e-4 | 0.002 |
| CoCl2 x 6 H2O | 1.8e-4 | 0.001 |
| Na2MoO4 | 1.6e-4 | 7.8e-4 |
| MnSO4 x H2O | 1.2e-4 | 7.1e-4 |
| H3BO3 | 1.1e-4 | 0.002 |
| Na2WO4 | 1.0e-4 | 3.4e-4 |
| AlCl3 | 4.0e-5 | 3.0e-4 |
| NiCl2 x 6 H2O | 2.5e-5 | 1.9e-4 |
| Distilled water | - | - |
Solutions:
- 1 ml FeEDTA solution
- 1 ml Trace element solution