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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

	Compound	Min. conc.	Max. conc.	Unit

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Current criteria: MOPS buffer

Your search received 3 results.

Compound	Conc. [g/L]	Conc. [mM]
Sulfur	9.98	311.195
MOPS buffer	3.892	18.6
Na-DL-lactate	2.495	22.265
Na₂CO₃	0.998	9.416
Na₂S x 9 H₂O	0.499	2.078
NH₄Cl	0.329	6.157
KCl	0.329	4.418
KH₂PO₄	0.329	2.42
CaCl₂ x 2 H₂O	0.329	2.24
MgCl₂ x 6 H₂O	0.329	1.62
Yeast extract	0.1	-
HCl	0.002	0.068
FeCl₂ x 4 H₂O	0.001	0.008
Sodium resazurin	5.0e-4	0.002
CoCl₂ x 6 H₂O	1.9e-4	0.001
MnCl₂ x 4 H₂O	1.0e-4	5.0e-4
Pyridoxine hydrochloride	1.0e-4	4.9e-4
ZnCl₂	7.0e-5	5.1e-4
Nicotinic acid	5.0e-5	4.1e-4
p-Aminobenzoic acid	5.0e-5	3.6e-4
(DL)-alpha-Lipoic acid	5.0e-5	2.4e-4
Calcium D-(+)-pantothenate	5.0e-5	2.1e-4
Thiamine HCl	5.0e-5	1.5e-4
Riboflavin	5.0e-5	1.3e-4
Na₂MoO₄ x 2 H₂O	3.6e-5	1.5e-4
NiCl₂ x 6 H₂O	2.4e-5	1.8e-4
Biotin	2.0e-5	8.2e-5
Folic acid	2.0e-5	4.5e-5
H₃BO₃	6.0e-6	9.7e-5
CuCl₂ x 2 H₂O	2.0e-6	1.2e-5
Vitamin B₁₂	1.0e-6	7.4e-7
Distilled water	-	-
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Solutions:

1 ml Trace element solution SL-10
1 ml Wolin's vitamin solution (10x)

Gas phase:

80 % N2
20 % CO2

Compound	Conc. [g/L]	Conc. [mM]
NaCl	112.613	1926.98
MgCl₂ x 6 H₂O	9.524	46.845
K₂SO₄	2.252	12.925
MOPS buffer	1.892	9.041
Na-acetate	1.802	21.964
NaHCO₃	1.111	13.226
Yeast extract	0.676	-
KH₂PO₄	0.541	3.972
NH₄Cl	0.45	8.421
CaCl₂ x 2 H₂O	0.19	1.296
Na₂S₂O₃ x 5 H₂O	0.09	0.363
HCl	0.002	0.062
FeCl₂ x 4 H₂O	0.001	0.007
CoCl₂ x 6 H₂O	1.7e-4	0.001
MnCl₂ x 4 H₂O	9.0e-5	4.6e-4
ZnCl₂	6.3e-5	4.6e-4
Na₂MoO₄ x 2 H₂O	3.2e-5	1.3e-4
NiCl₂ x 6 H₂O	2.2e-5	1.7e-4
H₃BO₃	5.4e-6	8.7e-5
CuCl₂ x 2 H₂O	1.8e-6	1.1e-5
Vitamin B₁₂	-	-
Distilled water	-	-
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Solutions:

13 ml Solution A
1 ml Solution B
0.2 ml Solution C
1 ml Trace element solution SL-10

Compound	Conc. [g/L]	Conc. [mM]
Sulfur	9.891	308.425
Na-acetate	4.946	60.287
MOPS buffer	2.967	14.18
Na₂S x 9 H₂O	0.495	2.059
NH₄Cl	0.326	6.102
KCl	0.326	4.378
KH₂PO₄	0.326	2.399
MgCl₂ x 6 H₂O	0.326	1.606
Yeast extract	0.099	-
MgSO₄ x 7 H₂O	0.03	0.12
Nitrilotriacetic acid	0.015	0.078
NaCl	0.01	0.169
MnSO₄ x H₂O	0.005	0.029
CoSO₄ x 7 H₂O	0.002	0.011
ZnSO₄ x 7 H₂O	0.002	0.006
CaCl₂ x 2 H₂O	9.9e-4	0.007
FeSO₄ x 7 H₂O	9.9e-4	0.004
Sodium resazurin	4.9e-4	0.002
NiCl₂ x 6 H₂O	3.0e-4	0.002
AlK(SO₄)₂ x 12 H₂O	2.0e-4	4.2e-4
H₃BO₃	9.9e-5	0.002
Pyridoxine hydrochloride	9.9e-5	4.8e-4
Na₂MoO₄ x 2 H₂O	9.9e-5	4.1e-4
CuSO₄ x 5 H₂O	9.9e-5	4.0e-4
Nicotinic acid	4.9e-5	4.0e-4
p-Aminobenzoic acid	4.9e-5	3.6e-4
(DL)-alpha-Lipoic acid	4.9e-5	2.4e-4
Calcium D-(+)-pantothenate	4.9e-5	2.1e-4
Thiamine HCl	4.9e-5	1.5e-4
Riboflavin	4.9e-5	1.3e-4
Biotin	2.0e-5	8.1e-5
Folic acid	2.0e-5	4.5e-5
Na₂WO₄ x 2 H₂O	4.0e-6	1.2e-5
Na₂SeO₃ x 5 H₂O	3.0e-6	1.1e-5
Vitamin B₁₂	9.9e-7	7.3e-7
Distilled water	-	-
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Solutions:

10 ml Modified Wolin's mineral solution
1 ml Wolin's vitamin solution (10x)

Gas phase:

80 % N2
20 % CO2

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480c DESULFURELLA (K-119) MEDIUM

747 DSIC MEDIUM (modified)

854 HIPPEA MEDIUM

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