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DSMZ

DSMZ 747 . DSIC MEDIUM (modified)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 747 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 13.0 ml - -
Solution B 1.0 ml - -
Solution C 0.2 ml - -
1 After cooling inject 1.0 ml of solution B and 0.2 ml of solution C in each tube of solution A. The pH of the complete medium is 7.0-7.1.

Solution A #

962
Equipment needed: Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 125.00 g 129.938 2223.44
NH4Cl 0.50 g 0.52 9.717
KH2PO4 0.60 g 0.624 4.583
K2SO4 2.50 g 2.599 14.913
Na-acetate 2.00 g 2.079 25.343
Na2S2O3 x 5 H2O 0.10 g 0.104 0.419
Yeast extract 0.75 g 0.78 -
Vitamin B12
(10 mg/100ml H2O) 		1.00 ml - -
Trace element solution SL-10 1.00 ml - -
MOPS buffer 2.10 g 2.183 10.432
Distilled water 960.00 ml - -
1 The acronym MOPS stands for the organic buffer N-morpholinopropanesulfonic acid at pH 7.0. The pH of solution A is 7.0. Boil the medium under N2 gas and distribute about 13 ml in 15 ml Hungate tubes. Autoclave at 121°C for 15 minutes.

Solution B #

70
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 10.0 g 142.857 702.681
CaCl2 x 2 H2O 0.2 g 2.857 19.434
Distilled water 70.0 ml - -
1 pH 7.0.
2 Boil medium under N2 gas and autoclave in a srew-capped bottle at 121°C for 15 minutes.

Solution C #

12
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 1 g 83.333 991.981
Distilled water 12 ml - -
1 Filter sterilize this solution.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 24.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.1
Equipment needed: Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 112.613 1926.98
MgCl2 x 6 H2O 9.524 46.845
K2SO4 2.252 12.925
MOPS buffer 1.892 9.041
Na-acetate 1.802 21.964
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain