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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

	Compound	Min. conc.	Max. conc.	Unit

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Search result

Current criteria: MgCl2 x 7 H2O

Your search received 7 results.

Compound	Conc. [g/L]	Conc. [mM]
Yeast extract	9.94	-
L-Arginine x HCl	2.087	-
L-Lysine HCl	1.789	-
Glycine	0.746	9.931
NH₄Cl	0.497	9.292
L-Cysteine HCl x H₂O	0.398	2.264
Na₂S x 9 H₂O	0.398	1.655
MgCl₂ x 7 H₂O	0.099	1.044
MgSO₄ x 7 H₂O	0.015	0.06
Nitrilotriacetic acid	0.007	0.039
NaCl	0.005	0.085
MnSO₄ x H₂O	0.002	0.015
CoSO₄ x 7 H₂O	8.9e-4	0.006
ZnSO₄ x 7 H₂O	8.9e-4	0.003
CaCl₂ x 2 H₂O	5.0e-4	0.003
Sodium resazurin	5.0e-4	0.002
FeSO₄ x 7 H₂O	5.0e-4	0.002
NiCl₂ x 6 H₂O	1.5e-4	0.001
Pyridoxine hydrochloride	9.9e-5	4.8e-4
AlK(SO₄)₂ x 12 H₂O	9.9e-5	2.1e-4
H₃BO₃	5.0e-5	8.0e-4
Nicotinic acid	5.0e-5	4.0e-4
p-Aminobenzoic acid	5.0e-5	3.6e-4
(DL)-alpha-Lipoic acid	5.0e-5	2.4e-4
Calcium D-(+)-pantothenate	5.0e-5	2.1e-4
Na₂MoO₄ x 2 H₂O	5.0e-5	2.1e-4
CuSO₄ x 5 H₂O	5.0e-5	2.0e-4
Thiamine HCl	5.0e-5	1.5e-4
Riboflavin	5.0e-5	1.3e-4
Biotin	2.0e-5	8.1e-5
Folic acid	2.0e-5	4.5e-5
Na₂WO₄ x 2 H₂O	2.0e-6	6.0e-6
Na₂SeO₃ x 5 H₂O	1.5e-6	5.7e-6
Vitamin B₁₂	9.9e-7	7.3e-7
Sodium Potassium phosphate buffer	-	20
Distilled water	-	-
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Solutions:

5 ml Modified Wolin's mineral solution
1 ml Wolin's vitamin solution (10x)

Gas phase:

100 % N2

Compound	Conc. [g/L]	Conc. [mM]
NaCl	20	342.231
Sodium pyruvate	3	27.263
MgCl₂ x 7 H₂O	1	10.503
NH₄Cl	0.6	11.217
KH₂PO₄	0.5	3.674
Yeast extract	0.4	-
CaCl₂ x 2 H₂O	0.15	1.02
Na₂-EDTA	0.005	0.015
FeCl₂ x 4 H₂O	0.002	0.008
CoCl₂ x 6 H₂O	1.9e-4	0.001
MnCl₂ x 4 H₂O	1.0e-4	5.1e-4
ZnCl₂	7.0e-5	5.1e-4
H₃BO₃	6.2e-5	0.001
Na₂MoO₄ x 2 H₂O	3.6e-5	1.5e-4
NiCl₂ x 6 H₂O	2.4e-5	1.9e-4
CuCl₂ x 2 H₂O	1.7e-5	1.0e-4
Distilled water	-	-
Vitamin B₁₂	-	-
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Solutions:

1 ml Micronutrient solution SL8

Compound	Conc. [g/L]	Conc. [mM]
Glucose	3.603	20
NaHCO₃	2	23.808
KNO₃	2	19.782
MgCl₂ x 6 H₂O	2	9.838
NH₄Cl	1	18.695
Yeast extract	1	-
MgCl₂ x 7 H₂O	0.5	5.252
Cysteine-HCl	0.5	3.172
Na₂S x 9 H₂O	0.4	1.665
KH₂PO₄	0.3	2.205
K₂HPO₄	0.3	1.722
KCl	0.1	1.341
MgSO₄ x 7 H₂O	0.03	0.122
Nitrilotriacetic acid	0.015	0.078
NaCl	0.01	0.171
MnSO₄ x H₂O	0.005	0.03
CoSO₄ x 7 H₂O	0.002	0.012
ZnSO₄ x 7 H₂O	0.002	0.006
CaCl₂ x 2 H₂O	1.0e-3	0.007
Resazurin	1.0e-3	0.004
FeSO₄ x 7 H₂O	1.0e-3	0.004
NiCl₂ x 6 H₂O	3.0e-4	0.002
AlK(SO₄)₂ x 12 H₂O	2.0e-4	4.2e-4
H₃BO₃	1.0e-4	0.002
Na₂MoO₄ x 2 H₂O	1.0e-4	4.1e-4
CuSO₄ x 5 H₂O	1.0e-4	4.0e-4
Na₂SeO₃ x 5 H₂O	3.0e-7	1.1e-6
Distilled water	-	-
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Solutions:

10 ml Trace Elements Solution

Compound	Conc. [g/L]	Conc. [mM]
Agar	20	59.465
CaCl₂ x 2 H₂O	7.351	50
MgCl₂ x 7 H₂O	5.1	53.566
Yeast extract	5	-
L-Proline	3	26.058
Distilled water	-	-

Compound	Conc. [g/L]	Conc. [mM]
Na₂CO₃	21.2	200.023
NaCl	20	342.231
Agar	20	59.465
NaHCO₃	13.44	159.987
Na-acetate	2	24.38
Na₂S₂O₃	1	2.334
Yeast extract	1	-
NH₄Cl	0.8	14.956
KH₂PO₄	0.8	5.879
MgCl₂ x 7 H₂O	0.1	1.05
CaCl₂ x 2 H₂O	0.05	0.34
FeCl₂ x 4 H₂O	0.002	0.009
H₃BO₃	5.0e-4	0.008
CoCl₂ x 6 H₂O	2.5e-4	0.002
ZnCl₂	1.0e-4	7.3e-4
MnCl₂ x 4 H₂O	7.0e-5	3.5e-4
Na₂MoO₄ x 2 H₂O	3.0e-5	1.2e-4
NiCl₂ x 6 H₂O	1.0e-5	7.7e-5
CuCl₂ x 2 H₂O	1.0e-5	5.9e-5
Na₂SeO₃ x 5 H₂O	1.0e-5	3.8e-5
Distilled water	-	-
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Solutions:

1 ml Trace element solution (A)

Compound	Conc. [g/L]	Conc. [mM]
NaCl	200	3422.31
MgCl₂ x 7 H₂O	40	420.122
Agar	40	118.931
K₂SO₄	5	28.693
Casamino acids	4	-
L-Glutamic acid	2	13.593
Trisodium citrate x 2 H₂O	2	6.8
Yeast extract	2	-
NH₄Cl	1	18.695
KH₂PO₄	1	7.348
FeSO₄	0.004	0.026
Na₂S₂O₃ x 5 H₂O	0.004	0.016
CaCl₂ x 2 H₂O	0.002	0.014
CoCl₂ x 6 H₂O	6.0e-4	0.005
NiCl₂ x 6 H₂O	2.0e-4	0.002
ZnCl₂	2.0e-4	0.001
MnCl₂ x 4 H₂O	2.0e-4	0.001
Na₂MoO₄ x 2 H₂O	2.0e-4	8.3e-4
AlCl3	8.0e-5	6.0e-4
Na₂WO₄ x 2 H₂O	4.0e-5	1.2e-4
H₃BO₄	4.0e-5	-
CuCl₂ x 6 H₂O	2.0e-5	1.5e-4
BaCl2 x 2 H₂O	2.0e-5	8.2e-5
Distilled water	-	-
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Solutions:

500 ml Mineral Salts Solution for solid Medium
500 ml Mineral Salts Solution for liquid Medium
1 ml Trace Element Solution
1 ml Trace Element Solution

Compound	Conc. [g/L]	Conc. [mM]
NaCl	19.92	340.868
MgCl₂ x 7 H₂O	3.984	41.845
Na-pyruvate	2.739	24.89
Na₂CO₃	1.494	14.096
CaCl₂ x 2 H₂O	0.996	6.775
Yeast extract	0.996	-
NH₄Cl	0.538	10.055
L-Cysteine HCl x H₂O	0.299	1.701
Na₂S x 9 H₂O	0.299	1.244
KH₂PO₄	0.1	0.732
HCl	0.002	0.068
FeCl₂ x 4 H₂O	0.001	0.008
NaOH	5.0e-4	0.012
Sodium resazurin	5.0e-4	0.002
Pyridoxine hydrochloride	2.0e-4	9.7e-4
CoCl₂ x 6 H₂O	1.9e-4	0.001
Nicotinic acid	1.0e-4	8.1e-4
p-Aminobenzoic acid	1.0e-4	7.3e-4
MnCl₂ x 4 H₂O	1.0e-4	5.0e-4
(DL)-alpha-Lipoic acid	1.0e-4	4.8e-4
Calcium D-(+)-pantothenate	1.0e-4	4.2e-4
Thiamine HCl	1.0e-4	3.0e-4
Riboflavin	1.0e-4	2.6e-4
ZnCl₂	7.0e-5	5.1e-4
Biotin	4.0e-5	1.6e-4
Folic acid	4.0e-5	9.0e-5
Na₂MoO₄ x 2 H₂O	3.6e-5	1.5e-4
NiCl₂ x 6 H₂O	2.4e-5	1.8e-4
H₃BO₃	6.0e-6	9.7e-5
Na₂WO₄ x 2 H₂O	4.0e-6	1.2e-5
Na₂SeO₃ x 5 H₂O	3.0e-6	1.1e-5
CuCl₂ x 2 H₂O	2.0e-6	1.2e-5
Vitamin B₁₂	2.0e-6	1.5e-6
Distilled water	-	-
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Solutions:

1 ml Trace element solution SL-10
1 ml Selenite-tungstate solution
2 ml Wolin's vitamin solution (10x)

Gas phase:

80 % N2
20 % CO2

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