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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Na2-glutamate
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371 NATRONOBACTERIA MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 200 | 3422.31 |
| Agar | 20 | 59.465 |
| Na2CO3 | 5 | 47.175 |
| Casamino acids | 5 | - |
| Yeast extract | 5 | - |
| NH4Cl | 1 | 18.695 |
| KCl | 1 | 13.414 |
| KH2PO4 | 1 | 7.348 |
| Na2-glutamate | 1 | 5.913 |
| MgSO4 x 7 H2O | 0.24 | 0.974 |
| CaSO4 x 2 H2O | 0.17 | 0.987 |
| HCl | 0.003 | 0.069 |
| FeCl2 x 4 H2O | 0.002 | 0.008 |
| CoCl2 x 6 H2O | 1.9e-4 | 0.001 |
| MnCl2 x 4 H2O | 1.0e-4 | 5.1e-4 |
| ZnCl2 | 7.0e-5 | 5.1e-4 |
| Na2MoO4 x 2 H2O | 3.6e-5 | 1.5e-4 |
| NiCl2 x 6 H2O | 2.4e-5 | 1.9e-4 |
| H3BO3 | 6.0e-6 | 9.7e-5 |
| CuCl2 x 2 H2O | 2.0e-6 | 1.2e-5 |
| Distilled water | - | - |
Solutions:
- 1 ml Trace element solution SL-10
729 MYX AGAR
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Na2-glutamate | 5 | 29.566 |
| Glucose | 2 | 11.101 |
| MgSO4 x 7 H2O | 1 | 4.057 |
| Yeast extract | 1 | - |
| Distilled water | - | - |