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Current criteria: Na2S x H2O
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1200 ANAEROMYXOBACTER-MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 4.545 | 54.108 |
| NaCl | 1.006 | 17.208 |
| MgCl2 x 6 H2O | 0.503 | 2.473 |
| Na-acetate | 0.402 | 4.904 |
| Disodium fumarate | 0.364 | 2.272 |
| NH4Cl | 0.302 | 5.64 |
| KCl | 0.302 | 4.047 |
| KH2PO4 | 0.201 | 1.478 |
| DL-Dithiothreitol | 0.14 | 0.907 |
| CaCl2 x 2 H2O | 0.015 | 0.103 |
| Na2S x H2O | 0.015 | 0.186 |
| L-Cysteine | 0.014 | 0.113 |
| HCl | 0.002 | 0.053 |
| FeSO4 x 7 H2O | 0.001 | 0.005 |
| NaOH | 0.001 | 0.025 |
| H3BO3 | 3.0e-4 | 0.005 |
| CoCl2 x 6 H2O | 1.9e-4 | 0.001 |
| Pyridoxine hydrochloride | 1.8e-4 | 8.8e-4 |
| MnCl2 x 4 H2O | 1.0e-4 | 5.0e-4 |
| Nicotinic acid | 9.1e-5 | 7.4e-4 |
| p-Aminobenzoic acid | 9.1e-5 | 6.6e-4 |
| Lipoic acid | 9.1e-5 | 4.4e-4 |
| Thiamine-HCl x 2 H2O | 9.1e-5 | 2.4e-4 |
| Riboflavin | 9.1e-5 | 2.4e-4 |
| D-Calcium pantothenate | 9.1e-5 | - |
| ZnCl2 | 7.0e-5 | 5.1e-4 |
| Biotin | 3.6e-5 | 1.5e-4 |
| Folic acid | 3.6e-5 | 8.2e-5 |
| Na2MoO4 x 2 H2O | 3.6e-5 | 1.5e-4 |
| NiCl2 x 6 H2O | 2.4e-5 | 1.8e-4 |
| Na2WO4 x 2 H2O | 8.0e-6 | 2.4e-5 |
| Na2SeO3 x 5 H2O | 6.0e-6 | 2.3e-5 |
| CuCl2 x 2 H2O | 2.0e-6 | 1.2e-5 |
| Vitamin B12 | 1.8e-6 | 1.3e-6 |
| Resazurin | - | - |
| Distilled water | - | - |
Solutions:
- 10 ml Solution A
- 1 ml Solution B
- 0.2 ml Solution C
- 0.2 ml Solution D ("red mix")
- 0.05 ml Solution E (electron acceptor)
- 0.2 ml Solution F: Vitamin solution according to Wolin and coauthors
- 1 ml Trace element solution SL-10 B
- 2 ml Selenite-tungstate solution
1516 THERMOFILUM MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 2 | 11.101 |
| NH4Cl | 0.33 | 6.169 |
| KCl | 0.33 | 4.427 |
| MgCl2 | 0.33 | 3.466 |
| CaCl2 | 0.33 | 2.974 |
| KH2PO4 | 0.33 | 2.425 |
| Na2S x H2O | 0.3 | 3.844 |
| Yeast extract | 0.1 | - |
| Fe(NH4)2(SO4)2 x 6 H2O | 7.8e-4 | - |
| NiCl2 x 2 H2O | 2.2e-4 | 0.002 |
| ZnSO4 x 7 H2O | 1.4e-4 | 5.0e-4 |
| Pyridoxine hydrochloride | 1.0e-4 | 4.9e-4 |
| MnCl2 x 4 H2O | 9.9e-5 | 5.0e-4 |
| Na2SeO4 | 9.5e-5 | 5.0e-4 |
| Nicotinic acid | 5.0e-5 | 4.1e-4 |
| p-Aminobenzoic acid | 5.0e-5 | 3.6e-4 |
| (DL)-alpha-Lipoic acid | 5.0e-5 | 2.4e-4 |
| Thiamine HCl | 5.0e-5 | 1.5e-4 |
| Riboflavin | 5.0e-5 | 1.3e-4 |
| Calcium D-(+)-pantothenate | 5.0e-5 | - |
| CoCl2 x 6 H2O | 3.8e-5 | 2.9e-4 |
| Na2WO4 x 2 H2O | 3.3e-5 | 1.0e-4 |
| Na2MoO4 x 2 H2O | 2.4e-5 | 9.9e-5 |
| Biotin | 2.0e-5 | 8.2e-5 |
| Folic acid | 2.0e-5 | 4.5e-5 |
| H3BO3 | 6.0e-6 | 9.7e-5 |
| CuCl2 x 2 H2O | 2.0e-6 | 1.2e-5 |
| Vitamin B12 | 1.0e-6 | 7.4e-7 |
| Distilled water | - | - |
Solutions:
- 10 ml Filtrate of Desulfurococcus kamchatkensis (DSM18924)
- 10 ml Wolin's vitamin solution
- 1000 ml Liquid medium
- 10 ml Liquid medium
- 1 ml Trace Element Solution