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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Na3-NTA x H2O
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195d KNH MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 20.875 | 357.2 |
| Na2SO4 | 2.982 | 20.995 |
| MgCl2 x 6 H2O | 2.982 | 14.668 |
| Na2CO3 | 1.491 | 14.068 |
| Na-acetate | 0.696 | 8.482 |
| KCl | 0.497 | 6.667 |
| Yeast extract | 0.497 | - |
| NH4Cl | 0.298 | 5.575 |
| KH2PO4 | 0.199 | 1.461 |
| CaCl2 x 2 H2O | 0.149 | 1.014 |
| Ascorbic acid | 0.099 | 0.564 |
| TiCl3 | 0.082 | 0.532 |
| HCl | 0.002 | 0.068 |
| FeCl2 x 4 H2O | 0.001 | 0.007 |
| NaOH | 5.0e-4 | 0.012 |
| Sodium resazurin | 5.0e-4 | 0.002 |
| CoCl2 x 6 H2O | 1.9e-4 | 0.001 |
| MnCl2 x 4 H2O | 9.9e-5 | 5.0e-4 |
| Pyridoxine hydrochloride | 9.9e-5 | 4.8e-4 |
| ZnCl2 | 7.0e-5 | 5.1e-4 |
| Nicotinic acid | 5.0e-5 | 4.0e-4 |
| p-Aminobenzoic acid | 5.0e-5 | 3.6e-4 |
| (DL)-alpha-Lipoic acid | 5.0e-5 | 2.4e-4 |
| Calcium D-(+)-pantothenate | 5.0e-5 | 2.1e-4 |
| Thiamine HCl | 5.0e-5 | 1.5e-4 |
| Riboflavin | 5.0e-5 | 1.3e-4 |
| Na2MoO4 x 2 H2O | 3.6e-5 | 1.5e-4 |
| NiCl2 x 6 H2O | 2.4e-5 | 1.8e-4 |
| Biotin | 2.0e-5 | 8.1e-5 |
| Folic acid | 2.0e-5 | 4.5e-5 |
| H3BO3 | 6.0e-6 | 9.6e-5 |
| Na2WO4 x 2 H2O | 4.0e-6 | 1.2e-5 |
| Na2SeO3 x 5 H2O | 3.0e-6 | 1.1e-5 |
| CuCl2 x 2 H2O | 2.0e-6 | 1.2e-5 |
| Vitamin B12 | 9.9e-7 | 7.3e-7 |
| Tris-HCl buffer | - | 0.093 |
| Na3-NTA x H2O | - | 0.031 |
| Show more | ||
Solutions:
- 952 ml Solution A
- 30 ml Solution B
- 10 ml Solution C
- 1 ml Solution D
- 13 ml Solution E
- 1 ml Selenite-tungstate solution
- 1 ml Trace element solution SL-10
- 1 ml Wolin's vitamin solution (10x)
Gas phase:
- 80 % H2
- 20 % CO2
1094 METHANOSPHAERULA (PEAT) MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| 2-(N-Morpholino)ethanesulfonic acid | 1.936 | 9.918 |
| Na-acetate | 0.407 | 4.963 |
| NaOH | 0.199 | 4.966 |
| TiCl3 | 0.082 | 0.531 |
| 2-Mercaptoethanesulfonic acid | 0.079 | 0.559 |
| Na2-EDTA | 0.037 | 0.11 |
| NH4Cl | 0.027 | 0.498 |
| KH2PO4 | 0.014 | 0.099 |
| Na2S x 9 H2O | 0.01 | 0.04 |
| AlK(SO4)2 x 12 H2O | 0.003 | 0.007 |
| CaCl2 x 2 H2O | 0.002 | 0.016 |
| MgSO4 x 7 H2O | 0.002 | 0.006 |
| KCl | 0.001 | 0.02 |
| FeCl2 x 4 H2O | 0.001 | 0.007 |
| Pyridoxine hydrochloride | 9.9e-5 | 4.8e-4 |
| ZnCl2 | 7.4e-5 | 5.5e-4 |
| Nicotinic acid | 5.0e-5 | 4.0e-4 |
| p-Aminobenzoic acid | 5.0e-5 | 3.6e-4 |
| (DL)-alpha-Lipoic acid | 5.0e-5 | 2.4e-4 |
| Calcium D-(+)-pantothenate | 5.0e-5 | 2.1e-4 |
| Thiamine HCl | 5.0e-5 | 1.5e-4 |
| Riboflavin | 5.0e-5 | 1.3e-4 |
| MnSO4 x 4 H2O | 2.6e-5 | 1.2e-4 |
| NiCl2 x 6 H2O | 2.4e-5 | 1.8e-4 |
| CoCl2 x 6 H2O | 2.4e-5 | 1.8e-4 |
| Na2Mo4 x 2 H2O | 2.4e-5 | - |
| Biotin | 2.0e-5 | 8.1e-5 |
| Folic acid | 2.0e-5 | 4.5e-5 |
| H3BO3 | 1.9e-5 | 3.1e-4 |
| CuSO4 x 5 H2O | 8.9e-6 | 3.6e-5 |
| Vitamin B12 | 9.9e-7 | 7.3e-7 |
| Tris-HCl buffer | - | 0.093 |
| Na3-NTA x H2O | - | 0.031 |
| Show more | ||
Solutions:
- 943 ml Solution A
- 13 ml Solution B
- 20 ml Solution C
- 1 ml Solution D
- 10 ml Solution E
- 10 ml Solution F
- 10 ml Solution G
- 1 ml Trace element solution
- 1 ml Wolin's vitamin solution (10x)
Gas phase:
- 40 % H2
- 40 % N2
- 20 % CO2
1656 HALANAEROBIUM HYDROGENIFORMANS MEDIUM (SL-HP)
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2CO3 | 2000 | 18870.1 |
| K2HPO4 | 315 | 1808.52 |
| Yeast extract | 50 | - |
| MgSO4 x 7 H2O | 1.471 | 5.966 |
| Na3-NTA x H2O | 0.799 | - |
| Show more | ||
Solutions:
- 10 ml Basal medium stock solution
- 10 ml Trace mineral solution
Gas phase:
- 80 % N2
- 20 % CO2