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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Sodium Potassium phosphate buffer

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643 SEDIMENTIBACTER MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 9.94 -
L-Arginine x HCl 2.087 -
L-Lysine HCl 1.789 -
Glycine 0.746 9.931
NH4Cl 0.497 9.292
L-Cysteine HCl x H2O 0.398 2.264
Na2S x 9 H2O 0.398 1.655
MgCl2 x 7 H2O 0.099 1.044
MgSO4 x 7 H2O 0.015 0.06
Nitrilotriacetic acid 0.007 0.039
NaCl 0.005 0.085
MnSO4 x H2O 0.002 0.015
CoSO4 x 7 H2O 8.9e-4 0.006
ZnSO4 x 7 H2O 8.9e-4 0.003
CaCl2 x 2 H2O 5.0e-4 0.003
Sodium resazurin 5.0e-4 0.002
FeSO4 x 7 H2O 5.0e-4 0.002
NiCl2 x 6 H2O 1.5e-4 0.001
Pyridoxine hydrochloride 9.9e-5 4.8e-4
AlK(SO4)2 x 12 H2O 9.9e-5 2.1e-4
H3BO3 5.0e-5 8.0e-4
Nicotinic acid 5.0e-5 4.0e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Calcium D-(+)-pantothenate 5.0e-5 2.1e-4
Na2MoO4 x 2 H2O 5.0e-5 2.1e-4
CuSO4 x 5 H2O 5.0e-5 2.0e-4
Thiamine HCl 5.0e-5 1.5e-4
Riboflavin 5.0e-5 1.3e-4
Biotin 2.0e-5 8.1e-5
Folic acid 2.0e-5 4.5e-5
Na2WO4 x 2 H2O 2.0e-6 6.0e-6
Na2SeO3 x 5 H2O 1.5e-6 5.7e-6
Vitamin B12 9.9e-7 7.3e-7
Sodium Potassium phosphate buffer - 20
Distilled water - -
Solutions:
  • 5 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 100 % N2

943 SEDIMENTIBACTER SAALENSIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.8 45.234
Yeast extract 3 -
L-Arginine x HCl 2.11 -
Glycine 0.75 9.991
(NH4)HCO3 0.45 -
Na2S x 9 H2O 0.15 0.625
MgSO4 x 6 H2O 0.13 1.08
CaCl2 x 2 H2O 0.12 0.816
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
NaOH 5.0e-4 0.013
Sodium resazurin 5.0e-4 -
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.4e-4
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
Calcium pantothenate 1.0e-4 2.1e-4
Vitamin B12 1.0e-4 7.4e-5
p-Aminobenzoic acid 8.0e-5 5.8e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.9e-4
D-(+)-biotin 2.0e-5 8.2e-5
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Sodium Potassium phosphate buffer - 10
Distilled water - -
Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2