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943. SEDIMENTIBACTER SAALENSIS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.45 g 0.444 5.614
2 0.12 g 0.118 0.805
3 0.13 g 0.128 0.561
4 Trace element solution SL-10 1.00 ml - -
5 Selenite-tungstate solution 1.00 ml - -
6 3.00 g 2.959 -
7 3.80 g 3.748 44.61
8
(0.1% w/v)
0.50 ml 4.9e-4 0.002
9
(1 M, pH 7.3)
10.00 ml - -
10 0.75 g 0.74 9.853
11 2.11 g 2.081 9.878
12 Seven vitamins solution 1.00 ml - -
13
(3% w/v)
0.15 g 0.148 0.616
14 1000.00 ml 984.425 54643.8
15 20.00 mg 0.02 -
Instructions:
  1. Dissolve ingredients (except bicarbonate, phosphate buffer, glycine, arginine, vitamins, and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add bicarbonate and adjust pH to 7.3, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add phosphate buffer, glycine, arginine, vitamins (sterilized by filtration), and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Prior to inoculation add 10 - 20 mg sodium dithionite per liter medium from a 5%(w/v) stock solution, freshly prepared under 100% N2 gas and filter-sterilized.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 987.368 54807.2
NaHCO3 3.748 44.61
Yeast extract 2.959 -
L-Arginine x HCI 2.081 9.878
Glycine 0.74 9.853
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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