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Main sol. 943

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Internal Solution-ID: S1940

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)HCO3 0.45 g 0.45 -
CaCl2 x 2 H2O 0.12 g 0.12 0.816
MgSO4 x 6 H2O 0.13 g 0.13 1.08
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Yeast extract 3.00 g 3 -
NaHCO3 3.80 g 3.8 45.234
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
Sodium Potassium phosphate buffer
(1 M, pH 7.3) 		10.00 ml - 10
Glycine 0.75 g 0.75 9.991
L-Arginine x HCl 2.11 g 2.11 -
Seven vitamins solution 1.00 ml - -
Na2S x 9 H2O
(3% w/v) 		0.15 g 0.15 0.625
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, phosphate buffer, glycine, arginine, vitamins, and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add bicarbonate and adjust pH to 7.3, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add phosphate buffer, glycine, arginine, vitamins (sterilized by filtration), and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Prior to inoculation add 10 - 20 mg sodium dithionite per liter medium from a 5% (w/v) stock solution, freshly prepared under 100% N2 gas and filter-sterilized.
943 SEDIMENTIBACTER SAALENSIS MEDIUM