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J933 THERMOANAEROBACULUM AQUATICUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sodium pyruvate 1.081 9.82
TES 0.982 4.285
Sodium nitrate 0.835 9.824
MgSO4 x 7 H2O 0.491 1.993
NH4Cl 0.334 6.244
KCl 0.334 4.48
KH2PO4 0.334 2.454
NaCl 0.246 4.202
CaCl2 x 2 H2O 0.246 1.67
Yeast extract 0.098 -
Nitrilotriacetic acid 0.002 0.01
Resazurin 9.8e-4 0.004
MnSO4 x n H2O 9.8e-4 -
Fe(NH4)2(SO4)2 x 6 H2O 7.9e-4 -
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 x 7 H2O 2.0e-4 6.8e-4
NiCl2 x 6 H2O 2.0e-5 1.5e-4
CuCl2 x 2 H2O 2.0e-5 1.2e-4
Na2SeO4 2.0e-5 1.0e-4
Na2MoO4 x 2 H2O 2.0e-5 8.1e-5
Na2WO4 x 2 H2O 2.0e-5 6.0e-5
Pyridoxine hydrochloride 9.8e-6 -
Nicotinic acid 4.9e-6 4.0e-5
p-Aminobenzoic acid 4.9e-6 3.6e-5
Lipoic acid 4.9e-6 2.4e-5
Riboflavin 4.9e-6 1.3e-5
Calcium pantothenate 4.9e-6 1.0e-5
Thiamine HCl 4.9e-6 -
Biotin 2.0e-6 8.0e-6
Folic acid 2.0e-6 4.5e-6
Vitamin B12 9.8e-8 7.2e-8
Na2S x 9 H2O - -
Coenzyme M - -
Distilled water - -
L-Cysteine HCl x H2O - -
Solutions:
  • 1 ml RST trace elements
  • 1 ml Trace vitamins