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J537 TREPONEMA ISOPTEROCOLA MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Yeast extract | 3.996 | - |
| Casein peptone | 1.998 | - |
| NaCl | 0.999 | 17.095 |
| KCl | 0.5 | 6.7 |
| MgCl2 x 6 H2O | 0.4 | 1.966 |
| NH4Cl | 0.3 | 5.603 |
| KH2PO4 | 0.2 | 1.468 |
| Na2SO4 | 0.15 | 1.055 |
| CaCl2 x 2 H2O | 0.1 | 0.68 |
| Resazurin | 5.0e-4 | 0.002 |
| Thiamine pyrophosphate | 2.5e-4 | 5.4e-4 |
| FeCl2 x 4 H2O | 7.5e-5 | 3.8e-4 |
| Pyridoxal phosphate | 2.5e-5 | 1.0e-4 |
| Pyridoxal hydrochloride | 2.5e-5 | - |
| Pyridoxine hydrochloride | 1.5e-5 | - |
| Nicotinic acid | 1.0e-5 | 8.1e-5 |
| Thiamine-HCl x 2 H2O | 1.0e-5 | - |
| CoCl2 x 6 H2O | 9.5e-6 | 7.3e-5 |
| Hemin | 6.5e-6 | 1.0e-5 |
| Calcium pantothenate | 5.0e-6 | 1.0e-5 |
| beta-NAD | 5.0e-6 | 7.5e-6 |
| Coenzyme A | 5.0e-6 | 6.5e-6 |
| FAD | 5.0e-6 | 6.4e-6 |
| Vitamin B12 | 5.0e-6 | 3.7e-6 |
| Ca-folinate | 5.0e-6 | - |
| MnCl2 x 4 H2O | 5.0e-6 | - |
| p-Aminobenzoic acid | 4.0e-6 | 2.9e-5 |
| ZnCl2 | 3.5e-6 | 2.6e-5 |
| Nicotinamide | 2.5e-6 | 2.0e-5 |
| Na2MoO4 x 2 H2O | 1.8e-6 | 7.4e-6 |
| NiCl2 x 6 H2O | 1.2e-6 | 9.3e-6 |
| D-(+)-biotin | 1.0e-6 | 4.1e-6 |
| H3BO3 | 3.0e-7 | 4.8e-6 |
| Folic acid | 2.5e-7 | 5.7e-7 |
| CuCl2 x 2 H2O | 1.0e-7 | 5.9e-7 |
| Riboflavin | 5.0e-8 | 1.3e-7 |
| HCl | - | - |
| Distilled water | - | - |
| NaHCO3 | - | - |
| Sugar | - | - |
| Dithiothreitol | - | - |
Solutions:
- 0.05 ml Trace element solution SL-10
- 0.05 ml Vitamin solution
- 0.1 ml Co-factors solution