1000 .
MJANHOX-NO3 MEDIUM WITH SUPPLEMENT
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Equipment needed:
Autoclave
Filter
Final pH:
7.9
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 3.000 | g | ||
|
|
K2HPO4 | 0.014 | g | ||
|
|
CaCl2 x 2 H2O | 0.014 | g | ||
|
|
NH4Cl | 0.040 | g | ||
|
|
MgSO4 x 7 H2O | 0.340 | g | ||
|
|
MgCl2 x 6 H2O | 0.418 | g | ||
|
|
KCl | 0.033 | g | ||
|
|
Fe2(SO4)3 x n H2O | 5.000 | mg | ||
|
|
NiCl2 x 6 H2O | 5.000 | µg | ||
|
|
Na2SeO3 x 5 H2O | 5.000 | µg | ||
|
|
Na2SiO3 x 9 H2O | 0.500 | g | ||
|
|
Trace mineral solution | 1.000 | ml | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 Before autoclaving, the pH of the Medium is adjusted with NaOH to 7.5-8.0. After autoclaving, the filter-sterilized vitamin solution (0.1% volume), and each of the separately autoclaved, concentrated solutions are added to the medium at a final concentration described below. Then a mix gas (80% H2, 20% CO2) is purged for 5 min. Finally, the mix gas is compressed into gas phase (> 80% volume of the tube or bottle) at 3 atm. | |||||
|
|
Na2S2O3 x 5 H2O | 10.000 | mM | ||
|
|
NaHCO3 | 0.200 | % | ||
|
|
NaNO3 | 0.050 | % | ||
Trace mineral solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g |
|
|
MnSO4 x 2 H2O | 0.50 | g |
|
|
CoSO4 x 7 H2O | 0.50 | g |
|
|
ZnSO4 x 7 H2O | 0.18 | g |
|
|
CuSO4 x 5 H2O | 0.01 | g |
|
|
AlK(SO4)2 x 12 H2O | 0.02 | g |
|
|
H3BO3 | 0.01 | g |
|
|
Na2MoO4 x 2 H2O | 0.01 | g |
|
|
Distilled water | 1000.00 | ml |