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1001. BASAL MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.50 g 2.5 29.76
2 0.25 g 0.25 4.674
3 0.60 g 0.6 4.348
4 0.10 g 0.1 1.341
5 Vitamin mixture 10.00 ml - -
6 Mineral mixture 10.00 ml - -
7 980.00 ml 978.243 54300.7
8 1.0 M Acetate Stock Solution x - -
9 500 mM Fe(III)NTA Stock solution x - -
Instructions:
  1. This medium should not be exposed to direct sunlight! Add all basal medium ingredients. Bring the final volume of the medium to 1.0 liter. Dispense to appropriate culture containers. Bubble the medium with 80:20 N2:CO2 (final pH sould be 6.8 to 7.0) - approximately 10 ml of media (anaerobic culture tube) should be gassed for 5 min in the aqueous phase (bubbled) and the headspace gassed for one minute prior to sealing the container. Sterilize per usual procedure. Add electron donor ( Acetate-final conc. of 10 mM-recipe below) and electron acceptor (Fe(III) NTA-final conc. of 10 mM-recipe below) from sterile, anaerobic stock solutions using a sterile syringe and needle flushed with anaerobic gas. Store out of direct light.
Equipment needed: Anaerobic workbench
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 13.6 g 136 1657.85
2 100.0 ml 998.207 55408.8
Instructions:
  1. Add 13.6 g sodium acetate to ca. 80 ml distilled water. Bring to final volume of 100 ml. Bubble with anaerobic gas (nitrogen) for 45 min. Seal and sterilize.
Equipment needed: Filter Anaerobic workbench
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 8.2 g 117.143 1394.45
2 70.0 ml 998.207 55408.8
3 12.8 g 182.857 -
4 13.5 g 192.857 713.503
5 10.0 N - -
Instructions:
  1. Add 8.2 g NaHCO3 to ca. 70 ml distilled water. Add 12.8 g of Na3Nitrilotriacetic acid ( NTA) to ca. 70 ml distilled water. Add 13.5 g FeCl3 x 6 H2O to ca 70 ml distilled water. Adjust pH to 6.5 using 10N NaOH. Bring solution to final volume of 100 ml. Ingredients will go into solution after stirring for ca. 15 min. Bubble with anaerobic gas (N2) for 45 min and then filter sterilize (0.2 μm filter) into a sterile, anaerobic, serum bottle.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.013
5 5.0 mg 0.005 -
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.023
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.500 g 1.5 -
2 3.000 g 3 24.923
3 0.500 g 0.5 2.958
4 1.000 g 1 17.111
5 0.100 g 0.1 0.36
6 0.100 g 0.1 0.68
7 0.100 g 0.1 0.42
8 0.130 g 0.13 0.954
9 0.010 g 0.01 0.04
10 0.010 g 0.01 0.021
11 0.010 g 0.01 0.162
12 0.025 g 0.025 0.121
13 0.024 g 0.024 0.101
14 0.025 g 0.025 0.076
15 1000.000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Anaerobic workbench, Filter

Associated strains

All strains for this medium: 1

Gas composition

  • 20 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
NaHCO3 2.5 29.76
NaH2PO4 x H2O 0.6 4.348
NH4Cl 0.25 4.674
KCl 0.1 1.341
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain

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