1012 .
PEREDIBACTER (DN) MEDIUM
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Equipment needed:
Autoclave
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nutrient broth
(BD Difco) |
0.8 | g | ||
|
|
Casamino acids
(BD Bacto) |
0.5 | g | ||
|
|
Yeast extract
(OXOID) |
0.1 | g | ||
|
|
CaCl2 x 2 H2O | 0.3 | g | ||
|
|
MgCl2 x 6 H2O | 0.6 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve ingredients except calcium and magnesium chloride and adjust pH of the medium to 7.2 with NaOH. After autoclaving add calcium and magnesium from sterile stock solutions. | |||||
| 2 Before inoculation prepare a suspension of prey cells in 30-40 ml medium using a 250 ml flask. Usually the resuspension of two overnight cultures of the prey strain on agar slants of DSMZ medium 54 will yield a final concentration of 108 to 109 cells per ml culture broth. | |||||