1021 .
SODALIS GLOSSINIDIUS MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Final pH:
8.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 900 | ml | ||
|
|
Solution B | 100 | ml | ||
| 1 Sterilize solution A and B separately at 121°C for 20 min. Cool to 50°C, then combine. To 4 parts of the above, add 1 part sterile foetal or new borne calf serum. Liquid medium should be filter sterilized instead of autoclaved. | |||||
Solution A #
900
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 6.20 | g | ||
|
|
Lactalbumin hydrolysate | 8.10 | g | ||
|
|
D-Glucose | 5.00 | g | ||
|
|
KCl | 0.25 | g | ||
|
|
MgCl2 x 6 H2O | 0.12 | g | ||
|
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
|
NaCl | 8.70 | g | ||
|
|
NaHCO3 | 0.15 | g | ||
|
|
Agar | 17.00 | g | ||
|
|
Distilled water | 900.00 | ml | ||
| 1 Adjust pH to 8.0. | |||||