1069 .
MODIFIED BIEBL AND PFENNIG'S MEDIUM
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Equipment needed:
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.50 | g | ||
|
|
CaCl2 x 2 H2O | 0.15 | g | ||
|
|
MgSO4 x 7 H2O | 2.00 | g | ||
|
|
NH4Cl | 0.34 | g | ||
|
|
NaCl | 20.00 | g | ||
|
|
Yeast extract | 0.40 | g | ||
|
|
Ferric citrate
(0.1% w/v) |
5.00 | ml | ||
|
|
Trace element solution SL-7 | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. | |||||
Trace element solution SL-7 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Distilled water | 1000 | ml |
|
|
HCl
(25%) |
1 | ml |
|
|
ZnCl2 | 70 | mg |
|
|
MnCl2 x 4 H2O | 100 | mg |
|
|
H3BO3 | 60 | mg |
|
|
CoCl2 x 6 H2O | 200 | mg |
|
|
CuCl2 x 2 H2O | 20 | mg |
|
|
NiCl2 x 6 H2O | 20 | mg |
|
|
Na2MoO4 x 2 H2O | 40 | mg |