1079 .
MYCOPLASMA/ACHOLEPLASMA MEDIUM (CIP MEDIUM 88)
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Equipment needed:
Autoclave
Final pH:
7.4 - 7.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
PPLO broth | 17 | g | ||
|
|
Yeast extract
(pH 7.0, autoclaved, 25%) |
100 | ml | ||
|
|
Phenol red
(pH 7.0, autoclaved, 1%) |
20 | ml | ||
|
|
Distilled water | 670 | ml | ||
|
|
Sterile supplement | 210 | ml | ||
| 1 Add all components except sterile supplement; adjust pH to 7.6 + 0.2. Autoclave at 121°C for 15 minutes; add aseptically sterile supplement. | |||||
Sterile supplement #
210
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glucose
(50 %) |
10 | ml | ||
|
|
Horse serum | 200 | ml | ||
|
|
Ampicillin | 1 | g | ||
| 1 Adjust pH to 7.0 + 0.2 and autoclave at 121°C for 15 minutes. | |||||