1080 .
MYCOPLASMA MEDIUM (CIP MEDIUM 89)
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Equipment needed:
Autoclave
Final pH:
7.0 - 7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
PPLO broth | 17 | g | ||
|
|
Yeast extract
(pH 7.0, autoclaved, 25%) |
100 | ml | ||
|
|
Phenol red
(pH 7.0, autoclaved, 1%) |
20 | ml | ||
|
|
Distilled water | 670 | ml | ||
|
|
Sterile supplement | 210 | ml | ||
| 1 Add all components except sterile supplement; adjust pH to 7.2 + 0.2. Autoclave at 121°C for 15 minutes; add aseptically sterile supplement. | |||||
Sterile supplement #
210
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Arginine solution 50%(w/v) | 10 | ml | ||
|
|
Horse serum | 200 | ml | ||
|
|
Ampicillin | 1 | g | ||
| 1 Autoclave at 121°C for 15 minutes. | |||||
Arginine solution 50%(w/v) #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
L-Arginine x HCl | 50 | g | ||
|
|
Distilled water | 100 | ml | ||
| 1 50 g L-Arginine hydrochloride plus distilled water to make up to 100 ml. | |||||