DSMZ 1081 . HORIKOSHI-1 MEDIUM

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Culture medium recipe

Vol.
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Medium for Streptomyces alkaliphilus DSM 42118

* Modification: pH 8


Growth conditions: 3-7 days aerobic 28 °C pH 8.00
Solution occurs in 1 other media (first defined in Medium 1081).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 10.0 g 10 55.506
Polypeptone 5.0 g 5 -
Yeast extract 5.0 g 5 -
K2HPO4 1.0 g 1 5.741
MgSO4 x 7 H2O 0.2 g 0.2 0.811
Agar 15.0 g 15 44.599
Distilled water 900.0 ml - -
Na2CO3
(10 %)
100.0 ml 10 94.35
1 After autoclaving, aseptically add 100 ml of a 10% Na2CO3 solution to the medium. Check final pH to be about 10.0.

Metadata

Last modified: 12.12.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 8.0
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Associated strains

All strains for this medium: 10
Strains with modifications: 2
Streptomyces alkaliphilus DSM 42118

  8.0

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2CO3 10 94.35
Glucose 10 55.506
Yeast extract 5 -
Polypeptone 5 -
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Cultivation metadata from 10 strains BacDive

halophily slightly halophilic 1 strain
pH 6 - 8 3 strains
pH 8 - 10 2 strains
phyla 2 10 strains
oxygen aerobe 3 strains
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