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1081. HORIKOSHI-1 MEDIUM

Culture medium recipe

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* Modified for Streptomyces alkaliphilus DSM 42118
beta

PH8

Solution occurs in 1 other media (first defined in medium 1081 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10.0 g 10 55.508
2 5.0 g 5 -
3 5.0 g 5 -
4 1.0 g 1 5.741
5 0.2 g 0.2 0.811
6 15.0 g 15 -
7 900.0 ml 898.386 49868
8 , required for pH adjustment
(10 %)
100.0 ml 25.3 238.706
Instructions:
  1. After autoclaving, aseptically add 100 ml of a 10% Na2CO3 solution to the medium. Check final pH to be about 10.0.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 10.0

Associated strains

All strains for this medium: 7
Strains with modifications: 2
Streptomyces alkaliphilus DSM 42118

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Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 898.386 49868
Na2CO3 25.3 238.706
Agar 15 -
Glucose 10 55.508
Yeast extract 5 -
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Cultivation metadata from 7 strains BacDive

halophily not 2 strains
halophily moderate 2 strains
halophily slightly 1 strain
halophily extreme 1 strain
pH 8 - 10 2 strains
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