1106
1106 .
SPOROSALIBACTERIUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1106 #
ml
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 40.0 | g | 40 | 684.463 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| KCl | 0.5 | g | 0.5 | 6.707 | |
| CaCl2 x 2 H2O | 0.2 | g | 0.2 | 1.36 | |
| Yeast extract | 2.0 | g | 2 | - | |
|
Trypticase peptone
(BD BBL) |
2.0 | g | 2 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| MgCl2 x 6 H2O | 3.0 | g | 3 | 14.756 | |
| Na2CO3 | 1.0 | g | 1 | 9.435 | |
| D-Glucose | 3.6 | g | 3.6 | 19.982 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except magnesium chloride, carbonate, glucose and reducing agents) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 -45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add magnesium chloride, glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The glucose solution should be sterilized by filtration. Adjust pH of complete medium to 6.8 - 7.0, if necessary. | |||||
Metadata
| Last modified: | 12.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 - 7.0 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 40 | 684.463 |
| D-Glucose | 3.6 | 19.982 |
| MgCl2 x 6 H2O | 3 | 14.756 |
| Yeast extract | 2 | - |
| Trypticase peptone | 2 | - |
| Show more | ||
