1107
1107 .
DIALISTER MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1107 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| DIALISTER MEDIUM | 10.00 | ml | - | - | |
| 1 To 10 ml of the dialister medium add aseptically: | |||||
|
Na-L-lactate
(50% (w/v)) |
0.10 | ml | 0.05 | - | |
|
Haemin
(0.5 mg/ml) |
0.10 | ml | - | - | |
|
NaHCO3
(10% (w/v)) |
0.10 | ml | 0.01 | 0.119 | |
|
Glucose
(40% (w/v)) |
0.10 | ml | 0.04 | 0.222 | |
|
Cystein-HCl x 2 H2O
(5% (w/v)) |
0.05 | ml | 0.003 | - | |
|
DTT
(100 mM) |
0.05 | ml | 7.7e-4 | 0.005 | |
| Vitamin K1 solution | 0.05 | ml | - | - | |
|
Na-formate
(6% (w/v)) |
0.50 | ml | 0.03 | 0.441 | |
|
Na-fumarate
(6% (w/v)) |
0.50 | ml | 0.03 | 0.187 | |
|
Horse blood
(sterile) |
0.50 | ml | - | - | |
| 2 Filter-sterilize all solutions and incubate them in oxygen-free atmosphere. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase
(BBL) |
17 | g | 17 | - | |
| Yeast extract | 3 | g | 3 | - | |
| NaCl | 3 | g | 3 | 51.335 | |
| KNO3 | 3 | g | 3 | 29.673 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| 2 Incubate in oxygen-free, 5 - 10% CO2 containing atmosphere. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin K1 | 0.1 | ml | - | - | |
|
Ethanol
(95 %) |
20.0 | ml | 19 | 412.416 | |
| 1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Gas composition
- 10 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Trypticase | 14.167 | - |
| NaCl | 2.5 | 42.779 |
| KNO3 | 2.5 | 24.727 |
| Yeast extract | 2.5 | - |
| Ethanol | 0.079 | 1.718 |
| Show more | ||
