Name: PHOTOTROPHIC MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1129 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.50	g	0.5	3.674
MgCl₂ x 6 H₂O	1.00	g	1	4.919
NaCl	20.00	g	20	342.231
NH₄Cl	0.60	g	0.6	11.217
CaCl₂ x 2 H₂O	0.15	g	0.15	1.02
Yeast extract	0.40	g	0.4	-
Ferric citrate (0.1%)	5.00	ml	0.005	0.02
Trace element solution SL7	1.00	ml	-	-
Distilled water	1000.00	ml	-	-
1 Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas.

Trace element solution SL7 #

1000

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%, v/v)	1	ml	-	-
ZnCl₂	70	mg	0.07	0.514
MnCl₂ x 4 H₂O	100	mg	0.1	0.505
H₃BO₃	60	mg	0.06	0.97
CoCl₂ x 6 H₂O	200	mg	0.2	1.54
CuCl₂ x 2 H₂O	20	mg	0.02	0.117
NiCl₂ x 6 H₂O	20	mg	0.02	0.154
Na₂MoO₄ x 2 H₂O	40	mg	0.04	0.165
Distilled water	1000	ml	-	-

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