DSMZ 1141 . THERMOGYMNOMONAS MEDIUM

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Equipment needed: Autoclave

Final pH: 3.0

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ml
Compound Amount Unit
(NH4)2SO4 0.20 g
KH2PO4 3.00 g
MgSO4 x 7 H2O 0.50 g
CaCl2 x 2 H2O 0.25 g
Yeast extract
(Difco)
1.00 g
Glucose 10.00 g
Distilled water 1000.00 ml
1 Adjust pH to 3.0 with 10 N H2SO4. Separately autoclave yeast extract and glucose as 10% solutions, and add aseptically to the medium.