Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
(NH4)2SO4 | 0.20 | g | ||
|
KH2PO4 | 3.00 | g | ||
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
Yeast extract
(Difco) |
1.00 | g | ||
|
Glucose | 10.00 | g | ||
|
Distilled water | 1000.00 | ml | ||
1 Adjust pH to 3.0 with 10 N H2SO4. Separately autoclave yeast extract and glucose as 10% solutions, and add aseptically to the medium. |