1146 .
VENENIVIBRIO STAGNISPUMANTIS MEDIUM
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Equipment needed:
Autoclave
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KCl | 0.50 | g | ||
|
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MgCl2 x 6 H2O | 1.36 | g | ||
|
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MgSO4 x 7 H2O | 7.00 | g | ||
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Na2S2O3 x 5 H2O | 2.00 | g | ||
|
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CaCl2 x 2 H2O | 0.40 | g | ||
|
|
NH4Cl | 0.20 | g | ||
|
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KH2PO4 | 0.25 | g | ||
|
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Trace mineral solution | 10.00 | ml | ||
|
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MES | 1.95 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Adjust the pH to 5.5 and gas the media with N2/CO2 (80/20) for 15 min at least. Fill 20 ml medium into 100 ml serum bottles, gas the flask containing the media with H2/CO2 (80/20) and seal with a rubber stopper. Autoclave the medium for 20 min at 121°C. After the inoculation the tubes are pressurized with H2/CO2/O2 (79%/15%/6%,170 kPa). | |||||
Trace mineral solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na-EDTA x 2 H2O | 500 | mg | ||
|
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CoCl2 x 6 H2O | 150 | mg | ||
|
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MnCl2 x 4 H2O | 100 | mg | ||
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FeSO4 x 7 H2O | 100 | mg | ||
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ZnCl2 | 100 | mg | ||
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AlCl3 x 6 H2O | 40 | mg | ||
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Na-tungstate x 2 H2O | 30 | mg | ||
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CuCl | 20 | mg | ||
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NiSO4 x 6 H2O | 20 | mg | ||
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Na2SeO3 | 10 | mg | ||
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H3BO3 | 10 | mg | ||
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Na2MoO4 x 2 H2O | 10 | mg | ||
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Distilled water | 1000 | ml | ||
| 1 Adjust the pH of the solution to 3.0 with HCl. | |||||