1152 .
PM MEDIUM
What is this page?
Equipment needed:
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 4.00 | g | ||
|
|
Na2HPO4 | 11.20 | g | ||
|
|
NaCl | 4.00 | g | ||
|
|
MgCl2 x 6 H2O | 0.10 | g | ||
|
|
MgSO4 x 7 H2O | 0.10 | g | ||
|
|
NH4Cl | 2.00 | g | ||
|
|
CaCl2 | 0.01 | g | ||
|
|
FeSO4 x 7 H2O | trace | µg | ||
|
|
Trace element solution SL-10 | 10.00 | ml | ||
|
|
Na acetate | 10.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
Agar , for solid medium | 20.00 | g | ||
| 1 Adjust to pH 7.2. The medium may be solidified by adding 20.0 g/l agar. | |||||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(25%; 7.7 M) |
10.0 | ml |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
ZnCl2 | 70.0 | mg |
|
|
MnCl2 x 4 H2O | 100.0 | mg |
|
|
H3BO3 | 6.0 | mg |
|
|
CoCl2 x 6 H2O | 190.0 | mg |
|
|
CuCl2 x 2 H2O | 2.0 | mg |
|
|
NiCl2 x 6 H2O | 24.0 | mg |
|
|
Na2MoO4 x 2 H2O | 36.0 | mg |
|
|
Distilled water | 990.0 | ml |