1154 .
GLUCOSE SULFIDE MEDIUM (Richard et al., 1985)
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Equipment needed:
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glucose | 0.150 | g | ||
|
|
(NH4)2SO4 | 0.500 | g | ||
|
|
CaCO3 | 0.100 | g | ||
|
|
Ca(NO3)2 | 0.100 | g | ||
|
|
KCl | 0.050 | g | ||
|
|
K2HPO4 | 0.050 | g | ||
|
|
MgSO4 x 7 H2O | 0.050 | g | ||
|
|
Na2S x 9 H2O | 0.187 | g | ||
|
|
Vitamin solution | 1.000 | ml | ||
|
|
Agar , for solid medium | 15.000 | g | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 Adjust to pH 7.3. Sterilize by autoclaving at 121°C for 15 minutes. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Biotin | 6.0 | µg | ||
|
|
Calcium pantothenate | 0.1 | µg | ||
|
|
Co-carboxylase | 0.1 | µg | ||
|
|
Cyanocobalamin | 6.0 | µg | ||
|
|
Folic acid | 6.0 | µg | ||
|
|
Inositol | 0.1 | µg | ||
|
|
Niacin | 0.1 | µg | ||
|
|
p-Aminobenzoic acid | 0.1 | µg | ||
|
|
Pyridoxine | 0.1 | µg | ||
|
|
Riboflavine | 0.1 | µg | ||
|
|
Thiamine | 0.1 | µg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 filter-sterilized, added after autoclaving | |||||