1155 .
SME MEDIUM
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Equipment needed:
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 27.70 | g | ||
|
|
MgSO4 x 7 H2O | 7.00 | g | ||
|
|
MgCl2 x 6 H2O | 5.50 | g | ||
|
|
KH2PO4 | 1.00 | g | ||
|
|
CaCl2 x 2 H2O | 0.75 | g | ||
|
|
KCl | 0.66 | g | ||
|
|
(NH4)2SO4 | 0.50 | g | ||
|
|
NaBr | 0.10 | g | ||
|
|
H3BO3 | 30.00 | mg | ||
|
|
SrCl2 x 6 H2O | 15.00 | mg | ||
|
|
KJ
(1 mg/ml) |
50.00 | µl | ||
|
|
Elemental sulfur | 10.00 | g | ||
|
|
Double distilled water | 1000.00 | ml | ||
|
|
Na2S
, used for reduction of the medium
(2.5 %) |
20.00 | ml | ||
|
|
Yeast extract | 0.04 | % | ||
| 1 Reduction of the medium by addition of 20 ml Na2S (2.5%); then adjust pH to 5.5 with sulfuric acid. | |||||
| 2 Gas phase: H2/CO2=80/20 (250 kPa). | |||||
| 3 After autoclaving add 0.04% yeast extract. | |||||
| 4 Incubation at 90°C for 1-2 days. | |||||