1176 .
HALOVIBRIO MEDIUM
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Equipment needed:
Autoclave
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 120.00 | g | ||
|
|
K2HPO4 | 3.00 | g | ||
|
|
NH4Cl | 0.50 | g | ||
|
|
Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | ||
|
|
HEPES | 2.38 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 After sterilization add from sterile stock solutions add: | |||||
|
|
MgSO4 x 7 H2O
(1M) |
2.00 | ml/l | ||
|
|
Sodium acetate
(2M) |
10.00 | ml/l | ||
|
|
Yeast extract
(10%) |
2.00 | ml/l | ||
| 2 Adjust pH to 7.5. Dispense the medium into sealed bottles and autoclave. | |||||
Trace elements solution (Pfennig & Lippert,1966) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 5.00 | g | ||
|
|
FeSO4 x 7 H2O | 2.20 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.03 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.03 | g | ||
|
|
NiCl2 x 6 H2O | 0.03 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 pH 3.0-4.0 | |||||