1187 .
ANOYXNATRONUM MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
9.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2CO3 | 25.0 | g | ||
|
|
NaHCO3 | 25.0 | g | ||
|
|
KCl | 0.2 | g | ||
|
|
MgCl2 x 6 H2O | 0.1 | g | ||
|
|
NH4Cl | 0.5 | g | ||
|
|
K2HPO4 | 0.2 | g | ||
|
|
Yeast extract | 0.2 | g | ||
|
|
Trace element solution SL-10 | 1.0 | ml | ||
|
|
Wolin's vitamin solution | 1.0 | ml | ||
|
|
Na2S x 9 H2O | 0.7 | g | ||
|
|
Glucose | 5.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 pH 9.0 | |||||
| 2 Prepare the medium anaerobically without the vitamins, Na2S, yeast extract NaHCO3, and Na2CO3. Boil the medium, cool under N2, add the NaHCO3, and Na2CO3, dispense under N2 and autoclave the medium. Add the vitamin solution, yeast extract, Na2S and glucose from sterile stock solution prepared under N2 to the cooled, autoclaved medium. | |||||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Wolin's vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium D-(+)-pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
(DL)-alpha-Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |