Name: RHODOBACTER /THIOCAPSA MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1197 #

Equipment needed: Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Yeast extract	0.40	g	0.4	-
Sodium pyruvate	3.00	g	3	27.263
KH₂PO₄	0.50	g	0.5	3.674
MgCl₂ x 6 H₂O	0.50	g	0.5	2.459
NaCl	0.40	g	0.4	6.845
NH₄Cl	0.60	g	0.6	11.217
CaCl₂ x 2 H₂O	0.05	g	0.05	0.34
Vitamin B₁₂ (2 mg/l filter sterilised)	1.00	ml	-	-
Trace element solution SL7	1.00	ml	-	-
Distilled water	1000.00	ml	-	-
1 pH 7.2
2 Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas.

Trace element solution SL7 #

1000

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%, v/v)	1	ml	-	-
ZnCl₂	70	mg	0.07	0.514
MnCl₂ x 4 H₂O	100	mg	0.1	0.505
H₃BO₃	60	mg	0.06	0.97
CoCl₂ x 6 H₂O	200	mg	0.2	1.54
CuCl₂ x 2 H₂O	20	mg	0.02	0.117
NiCl₂ x 6 H₂O	20	mg	0.02	0.154
Na₂MoO₄ x 2 H₂O	40	mg	0.04	0.165
Distilled water	1000	ml	-	-
1 Add the following component after autoclaving from sterile stock solutions:

All strains for this medium:

No strains found

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