This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 0.4 | g | 0.4 | 7.478 | |
KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
Na2SO4 | 0.5 | g | 0.5 | 3.52 | |
NaCl | 2.0 | g | 2 | 34.223 | |
KCl | 0.5 | g | 0.5 | 6.707 | |
NaHCO3 | 5.0 | g | 5 | 59.519 | |
Na2CO3 | 0.5 | g | 0.5 | 4.718 | |
Na2S2O3 x 5 H2O | 1.0 | g | 1 | 4.029 | |
Vitamin B12 | 10.0 | µg | 1.0e-5 | 7.4e-6 | |
Sodium pyruvate | 1.0 | g | 1 | 9.088 | |
Sodium acetate | 1.0 | g | 1 | 12.191 | |
Peptone | 1.0 | g | 1 | - | |
Yeast extract | 1.0 | g | 1 | - | |
Trace element solution SL10 | 1.0 | ml | - | - | |
Distilled water | 1000.0 | ml | - | - | |
1 Autoclave the NaHCO3 and Na2CO3 separately and add to the medium. Add the vitamin B12 solution from a filter sterilised stock solution to the autoclaved, cooled medium. Final is pH 8.5. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(concentrated) |
5.000 | ml | - | - |
FeCl2 x 4 H2O | 1.500 | g | 1.493 | 7.507 |
ZnCl2 | 0.070 | g | 0.07 | 0.511 |
MnCl2 x 4 H2O | 0.200 | g | 0.199 | 1.006 |
H3BO3 | 6.000 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 0.190 | g | 0.189 | 1.456 |
CuCl2 x 2 H2O | 2.000 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 0.024 | g | 0.024 | 0.184 |
Na2MoO4 x 2 H2O | 0.036 | g | 0.036 | 0.148 |
Distilled water | 1000.000 | ml | - | - |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 8.5 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaHCO3 | 5 | 59.519 |
NaCl | 2 | 34.223 |
Sodium acetate | 1 | 12.191 |
Sodium pyruvate | 1 | 9.088 |
Na2S2O3 x 5 H2O | 1 | 4.029 |
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