1218 .
ROSEINATRONOBACTER MONICUS MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
9.0 - 9.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NH4Cl | 0.4 | g | ||
|
|
KH2PO4 | 0.5 | g | ||
|
|
MgCl2 x 6 H2O | 0.2 | g | ||
|
|
Na2SO4 | 0.5 | g | ||
|
|
NaNO3 | 0.4 | g | ||
|
|
NaCl | 40.0 | g | ||
|
|
KCl | 0.5 | g | ||
|
|
NaHCO3 | 10.0 | g | ||
|
|
Na2CO3 | 5.0 | g | ||
|
|
Na2S2O3 x 5 H2O | 1.0 | g | ||
|
|
Vitamin B12 | 10.0 | µg | ||
|
|
Sodium pyruvate | 1.0 | g | ||
|
|
Sodium acetate | 1.0 | g | ||
|
|
Peptone | 1.0 | g | ||
|
|
Yeast extract | 1.0 | g | ||
|
|
Trace element solution SL10 | 1.0 | ml | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Autoclave the NaHCO3 and Na2CO3 separately and add to the medium. Add the vitamin B12 solution from a filter sterilised stock solution to the autoclaved, cooled medium. Final is pH 9.0 - 9.5 | |||||
Trace element solution SL10 #
1005
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(concentrated) |
5.000 | ml |
|
|
FeCl2 x 4 H2O | 1.500 | g |
|
|
ZnCl2 | 0.070 | g |
|
|
MnCl2 x 4 H2O | 0.200 | g |
|
|
H3BO3 | 6.000 | mg |
|
|
CoCl2 x 6 H2O | 0.190 | g |
|
|
CuCl2 x 2 H2O | 2.000 | mg |
|
|
NiCl2 x 6 H2O | 0.024 | g |
|
|
Na2MoO4 x 2 H2O | 0.036 | g |
|
|
Distilled water | 1000.000 | ml |