1222 .
RHODOVULUM MARINUM MEDIUM
What is this page?
Equipment needed:
Filter
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.50 | g | ||
|
|
NaCl | 20.00 | g | ||
|
|
NH4Cl | 0.60 | g | ||
|
|
CaCl2 x 2 H2O | 0.15 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
Sorbitol | 3.00 | g | ||
|
|
Trace element solution SL-8 | 1.00 | ml | ||
|
|
Vitamin B12
(20mg/l) |
1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Prepare the medium under a nitrogen atmosphere, dispense into tubes or bottles fitted with rubber stoppers so that they are half full. Add the vitamin B12 from a filter-sterilised stock solution. Either sorbitol or sodium pyruvate may serve as the carbon source. The final pH of the medium should be 6.8. | |||||
Trace element solution SL-8 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Distilled water | 1000.0 | ml | ||
|
|
Na2-EDTA | 5.2 | g | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
MnCl2 x 2 H2O | 100.0 | mg | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
H3BO3 | 62.0 | mg | ||
|
|
CuCl2 x 2 H2O | 17.0 | mg | ||
| 1 Adjust pH to 6.8. | |||||