1233
1233 .
CALDICOPROBACTER MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1233 #
ml
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 0.50 | g | 0.497 | 8.496 | |
| KCl | 0.20 | g | 0.199 | 2.664 | |
| MgCl2 x 6 H2O | 0.18 | g | 0.179 | 0.879 | |
| NH4Cl | 0.10 | g | 0.099 | 1.857 | |
|
HEPES
(SIGMA) |
4.80 | g | 4.767 | 20.003 | |
| Na2HPO4 x 7 H2O | 54.00 | mg | 0.054 | 0.378 | |
|
Fe(NH4)2(SO4)2 x 6 H2O
(0.1% w/v) |
4.00 | ml | 0.004 | - | |
|
CaCl2 x 2 H2O
(0.1% w/v) |
2.00 | ml | 0.002 | 0.014 | |
|
MnCl2 x 4 H2O
(0.1% w/v) |
1.00 | ml | 9.9e-4 | 0.005 | |
|
Yeast extract
(OXOID) |
5.00 | g | 4.965 | - | |
|
Tryptone
(BD Bacto) |
2.50 | g | 2.483 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 1.00 | g | 0.993 | 5.654 | |
| D-Glucose | 5.00 | g | 4.965 | 27.56 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine and glucose), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid cysteine and adjust pH to 7.3 - 7.5, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before inoculation, add glucose from a sterile anoxic stock solution prepared under 100% N2 gas. | |||||
| 2 Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) can be used to completely reduce the medium and may stimulate growth at the beginning. | |||||
Metadata
| Last modified: | 18.10.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.3 - 7.5 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| D-Glucose | 4.965 | 27.56 |
| Yeast extract | 4.965 | - |
| HEPES | 4.767 | 20.003 |
| Tryptone | 2.483 | - |
| L-Cysteine HCl x H2O | 0.993 | 5.654 |
| Show more | ||
