1276 .
MEDIUM FOR CHELATOCOCCUS BUHARII
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Equipment needed:
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 2.50 | g | ||
|
|
Tryptone | 2.50 | g | ||
|
|
Nitrilotriacetate
(NTA) |
1.00 | g | ||
|
|
K2HPO4 | 0.27 | g | ||
|
|
NaH2PO4 x 2 H2O | 0.54 | g | ||
|
|
MgSO4 x 7 H2O | 2.00 | g | ||
|
|
CaSO4 x 2 H2O | 0.40 | g | ||
|
|
Fe(III) citrate | 0.50 | g | ||
|
|
Trace element solution SL-6 | 5.00 | ml | ||
|
|
Agar | 18.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Before adding agar, heat to boiling. After cooling adjust pH to pH 7.5. (pH drops strongly during heating). | |||||
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
|
MnCl2 x 4 H2O | 0.03 | g |
|
|
H3BO3 | 0.30 | g |
|
|
CoCl2 x 6 H2O | 0.20 | g |
|
|
CuCl2 x 2 H2O | 0.01 | g |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
Na2MoO4 x 2 H2O | 0.03 | g |
|
|
Distilled water | 1000.00 | ml |