1279 .
HELICOBACTER MEDIUM
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Equipment needed:
Autoclave
Hungate tubes
Final pH:
5.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Brucella Broth
(Becton Dickinson) |
2.80 | g | ||
|
|
Demineralized water | 80.00 | ml | ||
| 1 Add aseptically to each tube: | |||||
|
|
Fetal bovine serum
(heat inactivated) |
2.00 | ml | ||
|
|
Vitox
(Oxoid) |
0.06 | ml | ||
|
|
Skirrow supplement
(Oxoid) |
0.02 | ml | ||
|
|
Amphotericin
(10 mg/ml) |
0.02 | ml | ||
| 2 Adjust with HCl to pH 5.0, make anoxic under a stream of N2/CO2, distribute in Hungate tubes to 8 ml and autoclave. | |||||
| 3 Add 1 ml of sterile air to each tube. | |||||