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DSMZ

DSMZ 1282 . SH SEAWATER MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1282 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 0.20 g 0.2 -
Yeast extract 0.10 g 0.1 -
NH4NO3 3.20 mg 0.003 -
NaCl 24.32 g 24.32 416.153
MgCl2 x 6 H2O 10.00 g 10 49.188
CaCl2 x 2 H2O 1.50 g 1.5 10.203
KCl 0.66 g 0.66 8.853
Na2SO4 4.00 g 4 28.161
Na2SO4 4.00 g 4 28.161
Trace element solution 1.00 ml - -
HEPES 2.38 g 2.38 9.987
KBr
(0.84M) 		1.00 ml 0.1 0.84
H3BO3
(0.4M) 		1.00 ml 0.025 0.4
SrCl2
(0.15M) 		1.00 ml 0.024 0.15
NH4Cl
(0.4M) 		1.00 ml 0.021 0.4
KH2PO4
(0.04M) 		1.00 ml 0.005 0.04
NaF
(0.07M) 		1.00 ml 0.003 0.07
Vitamin solution 1.00 ml - -
NaHCO3 0.20 g 0.2 2.381
Distilled water 1000.00 ml - -
Agar , for solid medium 15.00 g 15 44.599
1 pH 7.5 - 8.5
2 To solid media add 15.0 g/l agar. Add the vitamin solution (filter sterilized) and the NaHCO3 (0.2 g in 10 ml H2O, filter sterilized) to the autoclaved, cooled medium.

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.000 ml 2.5 68.568
FeCl2 x 4 H2O 1.500 g 1.5 7.545
CoCl2 x 6 H2O 0.190 g 0.19 1.463
MnCl2 x 2 H2O 0.100 g 0.1 -
ZnCl2 0.036 g 0.036 0.264
Na2MoO4 x 2 H2O 10.000 g 10 41.331
NiCl2 x 6 H2O 0.024 g 0.024 0.185
H3BO3 0.600 mg 6.0e-4 0.01
CuCl2 x 2 H2O 0.200 mg 2.0e-4 0.001
Double distilled water 990.000 ml - -

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Para-aminobenzoic acid 25 mg 0.025 0.182
Folic acid 10 mg 0.01 0.023
Biotin 10 mg 0.01 0.041
Nicotinic acid 25 mg 0.025 0.203
Calcium pantothenate 25 mg 0.025 0.052
Riboflavin 25 mg 0.025 0.066
Thiamine hydrochloride 25 mg 0.025 0.074
Pyridoxine hydrochloride 25 mg 0.025 0.122
Cyanocobalamine 25 mg 0.025 0.018
Lipoic acid 25 mg 0.025 0.121
Double distilled water 1000 ml - -

Metadata

Last modified: 31.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5 - 8.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 24.32 416.153
Agar 15 44.599
MgCl2 x 6 H2O 10 49.188
Na2SO4 4 28.161
HEPES 2.38 9.987
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Cultivation metadata from 1 strains BacDive

pH 8 - 10 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
temperature psychrophilic 1 strain