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DSMZ

DSMZ 1313 . MARINE AMMONIUM MINERAL SALTS

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1313 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 900 ml - -
Solution B 100 ml - -
Vitamin Solution V10 1 ml - -
1 Autoclave solution A and B separately and combine after cooling. Add 1ml/litre of filter sterilized vitamin solution V10

Solution A #

900
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 22.222 380.257
(NH4)2SO4 1.00 g 1.111 8.409
CaCl2 x 2 H2O 0.20 g 0.222 1.512
MgSO4 x 7 H2O 1.00 g 1.111 4.508
FeSO4 x 7 H2O 2.00 mg 0.002 0.008
Na2WO4 x 2 H2O 3.00 mg 0.003 0.01
Na2MoO4 x 2 H2O 0.02 g 0.022 0.092
Trace element solution SL10 1.00 ml - -
Distilled water 900.00 ml - -
1 Adjust the pH to 7.2 if needed with NaOH/HCl

Solution B #

100
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.36 g 3.6 26.454
K2HPO4 2.34 g 23.4 134.347
Distilled water 100.00 ml - -

Trace element solution SL10 #

1005
Solution occurs in 2 other media (first defined in Medium 951).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(concentrated) 		5.000 ml - -
FeCl2 x 4 H2O 1.500 g 1.493 7.507
ZnCl2 0.070 g 0.07 0.511
MnCl2 x 4 H2O 0.200 g 0.199 1.006
H3BO3 6.000 mg 0.006 0.097
CoCl2 x 6 H2O 0.190 g 0.189 1.456
CuCl2 x 2 H2O 2.000 mg 0.002 0.012
NiCl2 x 6 H2O 0.024 g 0.024 0.184
Na2MoO4 x 2 H2O 0.036 g 0.036 0.148
Distilled water 1000.000 ml - -

Vitamin Solution V10 #

1000
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Para-aminobenzoic acid 0.30 g 0.3 2.188
Biotin 0.02 g 0.02 0.082
Nicotinic acid 0.20 g 0.2 1.625
Calcium pantothenate 0.10 g 0.1 0.21
Pyridoxine hydrochloride 0.50 g 0.5 2.431
Lipoic acid 0.05 g 0.05 0.242
Folic acid 0.05 g 0.05 0.113
Cyanocobalamin 0.10 g 0.1 0.074
Thiamine 0.20 g 0.2 0.754
Riboflavin 0.10 g 0.1 0.266
Distilled water 1000.00 ml - -
1 Filter sterilize and store in the dark at 4°C.

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
K2HPO4 2.34 13.435
(NH4)2SO4 1 7.568
MgSO4 x 7 H2O 1 4.057
KH2PO4 0.36 2.645
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains