DSMZ 1320 . HALF STRENGTH R2A MEDIUM IN 75% SEAWATER

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Cerasicoccus frondis DSM 22189

This strain uses the standard medium without modifications.


Growth conditions: 28 °C
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 830 , prepare half strength medium with 75 % seawater 1000 ml - -
1 Refer to the composition of DSMZ medium 830 and either make up the medium in 2 litres of 75% seawater or use half of the amounts of the components specified and make up the medium in 1 litre of 75% seawater. Liquid medium can be prepared by leaving out the agar.
Solution occurs in 9 other media (first defined in Medium 830).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.50 g - -
Proteose peptone
(Difco no. 3)
0.50 g - -
Casamino acids 0.50 g - -
Glucose 0.50 g - -
Starch
(soluble)
0.50 g - -
Na-pyruvate 0.30 g - -
K2HPO4 0.30 g - -
MgSO4 x 7 H2O 0.05 g - -
Agar 15.00 g - -
Distilled water 1000.00 ml - -
1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3
Cerasicoccus frondis DSM 22189

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Glucose 0.5 2.775
Yeast extract 0.5 -
Proteose peptone 0.5 -
Casamino acids 0.5 -
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 3 strains
oxygen aerobe 1 strain
temperature mesophilic 2 strains
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